Journal of Fluorescence

, Volume 17, Issue 3, pp 339–343

A New Spectrofluorimetric Method for Determination of Trace Amounts 5-Hydroxytryptamine in Human Urine and Serum

Original Paper

DOI: 10.1007/s10895-007-0177-1

Cite this article as:
Peng, Q. & Jiang, C. J Fluoresc (2007) 17: 339. doi:10.1007/s10895-007-0177-1


A new spectrofluorimetric method was developed for the determination of trace amount of 5-hydroxytryptamine (5-HT) in human urine and serum samples. In the NaAc-HAc buffer solution of pH=5.80, 5-HT can react with formaldehyde-acetylacetone system to form a new compound which sends yellow green fluorescence at 533nm and the enhanced fluorescence intensity is in proportion to the concentration of 5-HT. Optimum conditions for the determination of 5-HT were also investigated. The dynamic range and detection limit for the determination of 5-HT are 5.35×10−7∼1.07×10−4 mol/L and 2.08×10−7 mol/L, respectively. The developed method is simple, practical and can be successfully applied to determination of 5-HT in human urine and serum samples. Moreover, the enhancement mechanisms of the fluorescence intensity in the 5-HT - formaldehyde-acetylacetone system have been also discussed.


5-hydroxytryptamine (5-HT)AcetylacetoneFormaldehydeSpectrofluorimetry

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.College of Chemistry, Chemical Engineering and Materials ScienceShandong Normal UniversityJinanChina