Journal of Fluorescence

, Volume 17, Issue 3, pp 279–287

Microwave-Triggered Chemiluminescence with Planar Geometrical Aluminum Substrates: Theory, Simulation and Experiment

Original Paper

DOI: 10.1007/s10895-007-0170-8

Cite this article as:
Previte, M.J.R. & Geddes, C.D. J Fluoresc (2007) 17: 279. doi:10.1007/s10895-007-0170-8

Abstract

Previously we combined common practices in protein detection with chemiluminescence, microwave technology, and metal-enhanced chemiluminescence to demonstrate that we can use low power microwaves to substantially increase enzymatic chemiluminescent reaction rates on particulate silvered substrates. We now describe the applicability of continuous aluminum metal substrates to potentially further enhance or “trigger” enzymatic chemiluminescence reactions. Furthermore, our results suggest that the extent of chemiluminescence enhancement for surface and solution based enzyme reactions critically depends on the surface geometry of the aluminum film.

In addition, we also use FDTD simulations to model the interactions of the incident microwave radiation with the aluminum geometries used. We demonstrate that the extent of microwave field enhancement for solution and surface based chemiluminescent reactions can be ascribed to “lightning rod” effects that give rise to different electric field distributions for microwaves incident on planar aluminum geometries. With these results, we believe that we can spatially and temporally control the extent of triggered chemiluminescence with low power microwave (Mw) pulses and maximize localized microwave triggered metal-enhanced chemiluminescence (MT-MEC) with optimized planar aluminum geometries. Thus we can potentially further improve the sensitivity of immunoassays with significantly enhanced signal-to-noise ratios.

Keywords

ImmunoassaysUltrasensitive assaysProtein detectionLow-power microwavesMetal-enhanced chemiluminescenceProtein quantificationPlasmonsPlasmonicsMetal-enhanced fluorescenceRadiative decay engineeringSurface enhanced fluorescencePlasmon enhanced fluorescencePlasmon enhanced luminescence

Acronyms and symbols

BSA

Bovine Serum Albumin

FDTD

Finite-Difference Time Domain

HRP

Horseradish peroxidase

MAMEF

Microwave-Accelerated Metal-Enhanced Fluorescence

MEF

Metal-Enhanced Fluorescence

MT-MEC

Microwave-Triggered Metal-Enhanced Chemiluminescence

Mw

Low-Power Microwave heating

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.Institute of Fluorescence, Laboratory for Advanced Fluorescence Spectroscopy & Laboratory for Advanced Medical Plasmonics, Medical Biotechnology CenterUniversity of Maryland Biotechnology InstituteBaltimoreUSA
  2. 2.Center for Fluorescence Spectroscopy, Medical Biotechnology CenterUniversity of Maryland School of MedicineBaltimoreUSA