Journal of Fluorescence

, Volume 16, Issue 4, pp 581–587

The Calibration Kit Spectral Fluorescence Standards—A Simple and Certified Tool for the Standardization of the Spectral Characteristics of Fluorescence Instruments

  • D. Pfeifer
  • K. Hoffmann
  • A. Hoffmann
  • C. Monte
  • U. Resch-Genger
Original Paper

DOI: 10.1007/s10895-006-0086-8

Cite this article as:
Pfeifer, D., Hoffmann, K., Hoffmann, A. et al. J Fluoresc (2006) 16: 581. doi:10.1007/s10895-006-0086-8

Abstract

With the Calibration Kit Spectral Fluorescence Standards BAM-F001–BAM-F005, we developed a simple tool for the characterization of the relative spectral responsivity and the long-term stability of the emission channel of fluorescence instruments under routine measurement conditions thereby providing the basis for an improved comparability of fluorescence measurements and eventually standardization. This first set of traceable fluorescence standards, which links fluorescence measurements to the spectral radiance scale in the spectral range of 300–770 nm and has been optimized for spectrofluorometers, can be employed for different measurement geometries and can be adapted to different fluorescence techniques with proper consideration of the underlying measurement principles.

Keywords

FluorescenceStandardSpectral correctionEmissionCalibration tool

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • D. Pfeifer
    • 1
  • K. Hoffmann
    • 1
  • A. Hoffmann
    • 1
  • C. Monte
    • 1
  • U. Resch-Genger
    • 1
  1. 1.Federal Institute for Materials Research and TestingWorking Group Optical Spectroscopy, Division I.5BerlinGermany