Journal of Chemical Ecology

, Volume 38, Issue 5, pp 538–546

Flavonoid Metabolites in the Hemolymph of European Pine Sawfly (Neodiprion sertifer) Larvae

Authors

    • Laboratory of Organic Chemistry and Chemical Biology, Department of ChemistryUniversity of Turku
  • Petri Tähtinen
    • Laboratory of Organic Chemistry and Chemical Biology, Department of ChemistryUniversity of Turku
  • Vladimir Ossipov
    • Laboratory of Organic Chemistry and Chemical Biology, Department of ChemistryUniversity of Turku
  • Juha-Pekka Salminen
    • Laboratory of Organic Chemistry and Chemical Biology, Department of ChemistryUniversity of Turku
Article

DOI: 10.1007/s10886-012-0113-y

Cite this article as:
Vihakas, M., Tähtinen, P., Ossipov, V. et al. J Chem Ecol (2012) 38: 538. doi:10.1007/s10886-012-0113-y

Abstract

Flavonoids in the hemolymph of European pine sawfly (Neodiprion sertifer) larvae that were feeding on Pinus sylvestris needles were identified. HPLC–ESI–MS analysis revealed that the main components in the hemolymph were flavonol di- and triglucosides and a catechin monoglucoside. These compounds were isolated from the larval hemolymph and their structures were established by HPLC–MS, GC–MS, and NMR spectroscopy. The isolated flavonoids were identified as (+)-catechin 7-O-β-glucoside, isorhamnetin 3,7,4′-tri-O-β-glucoside, kaempferol 3,7,4′-tri-O-β-glucoside, and quercetin 3,7,4′-tri-O-β-glucoside. The combined concentration of these four compounds in the hemolymph was 3.7 mg/ml. None of these compounds was present in the needles of P. sylvestris. Therefore, we propose that the flavonoid glucosides were produced by the larvae from flavonoid monoglucosides and (+)-catechin obtained from the pine needles.

Keywords

European pine sawflyScots pineHemolymphFlavonoid glycosidesHymenopteraDiprionidae

Supplementary material

10886_2012_113_MOESM1_ESM.doc (122 kb)
ESM 1(DOC 122 kb)

Copyright information

© Springer Science+Business Media, LLC 2012