Journal of Chemical Ecology

, Volume 38, Issue 3, pp 287–294

Knockdown of Microplitis mediator Odorant Receptor Involved in the Sensitive Detection of Two Chemicals

  • Ke-Ming Li
  • Li-Yan Ren
  • Yong-Jun Zhang
  • Kong-Ming Wu
  • Yu-Yuan Guo
Article

DOI: 10.1007/s10886-012-0085-y

Cite this article as:
Li, KM., Ren, LY., Zhang, YJ. et al. J Chem Ecol (2012) 38: 287. doi:10.1007/s10886-012-0085-y

Abstract

Odorant receptors are thought to play critical roles in the perception of chemosensory stimuli by insects. The primary method to address the functions of odorant receptors in insects is to use in vitro binding assays between the receptors and potential chemical stimuli. We injected MmedOrco dsRNA into the abdominal cavity of a braconid wasp, Microplitis mediator, and assayed for expression of this gene 72 h after treatment (RNAi). Quantitative real-time PCR demonstrated that the level of mRNA expression in MmedOrco dsRNA-treated M. mediator was significantly reduced (>90%) when compared with water-treated controls. Furthermore, electroantennogram (EAG) responses of M. mediator to two chemical attractants, nonanal and farnesene, were also reduced significantly (~70%) in RNAi-treated M. mediator when compared to controls. RNAi-treated M. mediator also responded by walking/flying at a lower rate to both chemicals when compared with controls in a Y-tube olfactometer bioassay, which provides direct evidence that MmedOrco plays an important role in perception of nonanal and farnesene in M. mediator.

Keywords

BraconidaeElectroantennogramHymenopteraMicroplitis mediatorOdorant receptorQuantitative real-time polymerase chain reaction (qPCR)Reverse transcription PCR (RT-PCR) analysisRNA interference (RNAi)Walking/flight behaviorY-tube bioassay

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Ke-Ming Li
    • 1
    • 2
  • Li-Yan Ren
    • 2
  • Yong-Jun Zhang
    • 2
  • Kong-Ming Wu
    • 2
  • Yu-Yuan Guo
    • 2
  1. 1.College of Plant ProtectionNorthwest A & F UniversityYanglingChina
  2. 2.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina