Journal of Chemical Ecology

, Volume 36, Issue 10, pp 1058–1067

Partitioning of New Carbon as 11C in Nicotiana tabacum Reveals Insight into Methyl Jasmonate Induced Changes in Metabolism

  • Nils Hanik
  • Sara Gómez
  • Marcel Best
  • Michael Schueller
  • Colin M. Orians
  • Richard A. Ferrieri
Article

DOI: 10.1007/s10886-010-9835-x

Cite this article as:
Hanik, N., Gómez, S., Best, M. et al. J Chem Ecol (2010) 36: 1058. doi:10.1007/s10886-010-9835-x

Abstract

We examined the timeline by which methyl jasmonate (MeJA) reprograms new carbon partitioning into key metabolite pools. The radioactive isotope 11C (t1/2 20.4 min), administered to intact leaves of Nicotiana tabacum L. (cv Samsun) as 11CO2 gas enabled us to measure changes in new carbon partitioning into soluble sugar and amino acid pools of [11C]photosynthate. A 500 μM MeJA treatment resulted in a decrease in the [11C]soluble sugar pool and an increase in the [11C]amino acid pool after 4 h. This pattern was more pronounced 15 h after treatment. We also examined the timeline for 11C-partitioning into aromatic amino acid metabolites of the shikimate pathway. [11C]Tyrosine, [11C]phenylalanine and [11C]tryptophan were elevated 1.5-fold, 12-fold and 12-fold, respectively, relative to controls, 4 h after MeJA treatment, while endogeneous pools were unchanged. This suggests that only new carbon is utilized during early stages of defense induction. By 15 h, [11C]tyrosine and [11C]phenylalanine returned to baseline while [11C]tryptophan was elevated 30-fold, suggesting that MeJA exerts selective control over the shikimate pathway. Finally, we measured trans-cinnamic acid levels as a gauge of downstream phenolic metabolism. Levels were unchanged 4 h after MeJA treatment relative to controls, but were increased 2-fold by 15 h, indicating a lag in response of secondary metabolism.

Key Words

Methyl jasmonatePlant defensesMetabolic partitioningChemical signalingShort-lived radiotracers11CNicotiana tabacumShikimate pathway

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Nils Hanik
    • 1
  • Sara Gómez
    • 2
    • 4
  • Marcel Best
    • 1
  • Michael Schueller
    • 3
  • Colin M. Orians
    • 4
  • Richard A. Ferrieri
    • 3
  1. 1.Fachbereich ChemieJohannes Gutenberg UniversitätMainzGermany
  2. 2.Department of Biological SciencesUniversity of Rhode IslandKingstonUSA
  3. 3.Medical DepartmentBrookhaven National LaboratoryUptonUSA
  4. 4.Department of BiologyTufts UniversityMedfordUSA