Journal of Chemical Ecology

, Volume 33, Issue 11, pp 2011–2024

Genetic and Environmental Sources of Variation in the Autogenous Chemical Defense of a Leaf Beetle

  • Y. Triponez
  • R. E. Naisbit
  • J. B. Jean-Denis
  • M. Rahier
  • N. Alvarez
Article

DOI: 10.1007/s10886-007-9351-9

Cite this article as:
Triponez, Y., Naisbit, R.E., Jean-Denis, J.B. et al. J Chem Ecol (2007) 33: 2011. doi:10.1007/s10886-007-9351-9

Abstract

Chemical defense plays a central role for many herbivorous insects in their interactions with predators and host plants. The leaf beetle genus Oreina (Coleoptera, Chrysomelidae) includes species able to both sequester pyrrolizidine alkaloids and autogenously produce cardenolides. Sequestered compounds are clearly related to patterns of host-plant use, but variation in de novo synthesized cardenolides is less obviously linked to the environment. In this study, intraspecific variation in cardenolide composition was examined by HPLC–MS analysis in 18 populations of Oreina speciosa spanning Europe from the Massif Central to the Balkans. This revealed the defense secretion to be a complex blend of up to 42 compounds per population. There was considerable geographical variation in the total sample of 50 compounds detected, with only 14 found in all sites. The environmental and genetic influences on defense chemistry were investigated by correlation with distance matrices based on habitat factors, host-plant use, and genetics (sequence data from COI, COII, and 16s rRNA). This demonstrated an influence of both genetics and host-plant use on the overall blend of cardenolides and on the presence of some of the individual compounds. The implications of this result are discussed for the evolution of defense chemistry and for the use of cardenolide compounds as markers of the evolutionary history of the species.

Keywords

ChrysomelidaeOreina speciosaChemical defenseCardenolidesIntraspecific variationHost-plant useGenetic constraintsHPLC–MS

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Y. Triponez
    • 1
  • R. E. Naisbit
    • 1
  • J. B. Jean-Denis
    • 2
  • M. Rahier
    • 1
  • N. Alvarez
    • 1
  1. 1.Laboratoire d’Entomologie Evolutive, Institut de BiologieUniversité de NeuchâtelNeuchâtelSwitzerland
  2. 2.Institut de ChimieUniversité de NeuchâtelNeuchâtelSwitzerland