Article

Journal of Chemical Ecology

, Volume 31, Issue 2, pp 377-392

First online:

Stir Bar Sorptive Extraction: A New Quantitative and Comprehensive Sampling Technique for Determination of Chemical Signal Profiles from Biological Media

  • Helena A. SoiniAffiliated withDepartment of Chemistry, Institute for Pheromone Research, Indiana University
  • , Kevin E. BruceAffiliated withDepartment of Chemistry, Institute for Pheromone Research, Indiana University
  • , Donald WieslerAffiliated withDepartment of Chemistry, Institute for Pheromone Research, Indiana University
  • , Frank DavidAffiliated withResearch Institute for Chromatography
  • , Pat SandraAffiliated withResearch Institute for Chromatography
  • , Milos V. NovotnyAffiliated withDepartment of Chemistry, Institute for Pheromone Research, Indiana University Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Various applications of a novel sampling procedure in chemical ecology are outlined. The stir bar extraction method features the analytical reproducibility needed in recording the analytical profiles of volatile and semivolatile components of biological mixtures. This methodology has been demonstrated here through the examples of small volume urine samples, glandular tissue volatiles, and the air blown through animal cages. Its analytical merits are compared with those of the previously established purge-and-trap (dynamic headspace) technique.

Keywords

Pheromones chemical signals hamster urine methods mouse urine rat preputial gland rat odor volatiles stir bar sorptive extraction gas chromatography–mass spectrometry