Journal of Chemical Ecology

, Volume 31, Issue 2, pp 377–392

Stir Bar Sorptive Extraction: A New Quantitative and Comprehensive Sampling Technique for Determination of Chemical Signal Profiles from Biological Media

Authors

  • Helena A. Soini
    • Department of Chemistry, Institute for Pheromone ResearchIndiana University
  • Kevin E. Bruce
    • Department of Chemistry, Institute for Pheromone ResearchIndiana University
  • Donald Wiesler
    • Department of Chemistry, Institute for Pheromone ResearchIndiana University
  • Frank David
    • Research Institute for Chromatography
  • Pat Sandra
    • Research Institute for Chromatography
    • Department of Chemistry, Institute for Pheromone ResearchIndiana University
Article

DOI: 10.1007/s10886-005-1347-8

Cite this article as:
Soini, H.A., Bruce, K.E., Wiesler, D. et al. J Chem Ecol (2005) 31: 377. doi:10.1007/s10886-005-1347-8

Abstract

Various applications of a novel sampling procedure in chemical ecology are outlined. The stir bar extraction method features the analytical reproducibility needed in recording the analytical profiles of volatile and semivolatile components of biological mixtures. This methodology has been demonstrated here through the examples of small volume urine samples, glandular tissue volatiles, and the air blown through animal cages. Its analytical merits are compared with those of the previously established purge-and-trap (dynamic headspace) technique.

Keywords

Pheromoneschemical signalshamster urinemethodsmouse urinerat preputial glandrat odorvolatilesstir bar sorptive extractiongas chromatography–mass spectrometry

Copyright information

© Springer Science + Business Media, Inc. 2005