Original Paper

Journal of Cluster Science

, Volume 21, Issue 3, pp 427-438

Antibacterial Activity of Light-Activated Silicone Containing Methylene Blue and Gold Nanoparticles of Different Sizes

  • Stefano PerniAffiliated withDivision of Microbial Diseases, UCL Eastman Dental Institute, University College London
  • , Clara PiccirilloAffiliated withDepartment of Chemistry, Materials Chemistry Research Centre, University College London
  • , Andreas KafizasAffiliated withDepartment of Chemistry, Materials Chemistry Research Centre, University College London
  • , Madeeha UppalAffiliated withDepartment of Chemistry, Materials Chemistry Research Centre, University College London
  • , Jonathan PrattenAffiliated withDivision of Microbial Diseases, UCL Eastman Dental Institute, University College London
  • , Mike WilsonAffiliated withDivision of Microbial Diseases, UCL Eastman Dental Institute, University College London
  • , Ivan P. ParkinAffiliated withDepartment of Chemistry, Materials Chemistry Research Centre, University College London Email author 

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Abstract

Methylene Blue and methylene blue-gold nanoparticle mixtures were encapsulated in a silicone polymer using a swell-encapsulation-shrink technique. The antibacterial properties of the materials, when tested against Escherichia coli and Staphylococcus epidermidis, and exposed to laser light (660 nm), were significantly affected by both the presence and size of Au nanoparticles. Bacterial inactivation data were analysed using the Weibull inactivation model. For both E. coli and S. epidermidis the value of the parameter, indicating the time required to achieve the first log10 reduction in the viable count, decreased when Au nanoparticles of ca 2 nm diameter were present. Larger Au nanoparticles (diameters of 5 and 20 nm) in combination with methylene blue were also embedded in silicone. The values of these materials increased with nanoparticle diameter, indicating a reduction in antibacterial activity. In all cases E. coli had higher values than S. epidermidis.

Keywords

E. coli S. epidermidis Gold nanoparticles Methylene blue Photodynamic therapy