KRN/I-Ag7 Mouse Arthritis Is Independent of Complement C3
- Patricia Y. TsaoAffiliated withDepartment of Medicine, Division of Rheumatology, University of Pennsylvania
- , Vaishali AroraAffiliated withDepartment of Medicine, Division of Rheumatology, University of Pennsylvania
- , Mei Qing JiAffiliated withDepartment of Medicine, Division of Rheumatology, University of Pennsylvania
- , Alexander C. WrightAffiliated withDepartment of Radiology, University of Pennsylvania Medical Center
- , Robert A. EisenbergAffiliated withDepartment of Medicine, Division of Rheumatology, University of Pennsylvania Email author
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KRN/I-Ag7 (KxB/N) is a mouse model of inflammatory arthritis, which resembles human rheumatoid arthritis. Arthritis in these animals is caused by autoreactivity to a ubiquitously expressed autoantigen, glucose-6 phosphate isomerase. Tolerance is broken at both the T cell and B cell level. The sera from KRN/I-Ag7 mice can induce mouse arthritis in healthy mice. Complement components of the alternative complement pathway, including C3, have been shown to be required in induction of mouse arthritis by serum transfer.
We have bred KRN/I-Ag7 mice onto a C3-deficient background and followed cohorts for the spontaneous appearance of arthritis. We have also transferred KxB/N serum to B6.I-A g7 recipients.
C3-deficient KRN/I-Ag7 mice spontaneously developed severe, destructive arthritis, comparable to that seen in C3-intact KRN/I-Ag7 mice. However, serum transfer experiments confirmed the strong requirement for C3 in the passive model.
The pathogenesis of spontaneous KRN/I-Ag7 arthritis can largely proceed by complement-independent pathways and must have pathology effector mechanisms in addition to those seen in the passive serum transfer model.
KeywordsArthritis autoantibody KRN GPI complement C3
- KRN/I-Ag7 Mouse Arthritis Is Independent of Complement C3
Journal of Clinical Immunology
- Online Date
- July 2011
- Print ISSN
- Online ISSN
- Springer US
- Additional Links
- complement C3
- Industry Sectors
- Author Affiliations
- 1. Department of Medicine, Division of Rheumatology, University of Pennsylvania, 756 BRB II/III, 421 Curie Blvd., Philadelphia, PA, 19104-6160, USA
- 2. Department of Radiology, University of Pennsylvania Medical Center, Philadelphia, PA, 19104, USA