Journal of Clinical Immunology

, Volume 31, Issue 3, pp 436–442

Serodiagnostic Assays for Celiac Disease Based on the Open or Closed Conformation of the Autoantigen, Transglutaminase 2

Authors

  • Katri Lindfors
    • Pediatric Research CenterUniversity of Tampere and Tampere University Hospital
  • Outi Koskinen
    • Pediatric Research CenterUniversity of Tampere and Tampere University Hospital
  • Kalle Kurppa
    • Pediatric Research CenterUniversity of Tampere and Tampere University Hospital
  • Kaija Laurila
    • Pediatric Research CenterUniversity of Tampere and Tampere University Hospital
  • Pekka Collin
    • Department of Gastroenterology and Alimentary Tract SurgeryTampere University Hospital
    • School of MedicineUniversity of Tampere
  • Katri Haimila
    • Finnish Red Cross Blood Service
  • Jukka Partanen
    • Finnish Red Cross Blood Service
  • Päivi Saavalainen
    • Department of Medical Genetics and Research Program for Molecular Medicine, Biomedicum HelsinkiUniversity of Helsinki
  • Markku Mäki
    • Pediatric Research CenterUniversity of Tampere and Tampere University Hospital
    • Department of Gastroenterology and Alimentary Tract SurgeryTampere University Hospital
    • School of MedicineUniversity of Tampere
Article

DOI: 10.1007/s10875-011-9514-x

Cite this article as:
Lindfors, K., Koskinen, O., Kurppa, K. et al. J Clin Immunol (2011) 31: 436. doi:10.1007/s10875-011-9514-x

Abstract

The autoantigen of celiac disease, transglutaminase 2 (TG2), adopts an open conformation during enzymatic activation. We studied diagnostic accuracy of serodiagnostic assays using TG2 in its open and closed conformation as antigens in patients with diagnostic difficulties. The open TG2 antibody (TG2ab) test identified 93% of untreated celiac patients in contrast to 44%, 27%, and 68% detected by closed and conventional TG2ab and endomysial antibody (EmA) tests, respectively. The assay was able to detect 60% of non-responding celiac patients seronegative for conventional TG2ab and EmA. The titers of the openTG2abs were higher than those of the closed TG2abs. The serological test utilizing TG2 in an open conformation was more accurate than the other assays in finding active celiac disease even in patients having negative or borderline conventional celiac autoantibodies and in revealing poor dietary response non-invasively. It thus offers a promising tool in the diagnostics and follow-up of celiac disease.

Keywords

Celiac diseaseglutentransglutaminase 2open conformationdiagnostic accuracy

Copyright information

© Springer Science+Business Media, LLC 2011