Journal of Clinical Immunology

, Volume 31, Issue 1, pp 69–73

Association Study of Common Genetic Variants in Pre-microRNAs in Patients with Ulcerative Colitis

  • Masaaki Okubo
  • Tomomitsu Tahara
  • Tomoyuki Shibata
  • Hiromi Yamashita
  • Masakatsu Nakamura
  • Daisuke Yoshioka
  • Joh Yonemura
  • Yoshio Kamiya
  • Takamitsu Ishizuka
  • Yoshihito Nakagawa
  • Mitsuo Nagasaka
  • Masami Iwata
  • Hideto Yamada
  • Ichiro Hirata
  • Tomiyasu Arisawa
Article

DOI: 10.1007/s10875-010-9461-y

Cite this article as:
Okubo, M., Tahara, T., Shibata, T. et al. J Clin Immunol (2011) 31: 69. doi:10.1007/s10875-010-9461-y

Abstract

Background

Common single-nucleotide polymorphisms (SNPs) in microRNAs (miRNA) have been shown to be associated with susceptibility to several human diseases. We evaluated the associations of three SNPs (rs11614913, rs2910164, and rs3746444) in pre-miRNAs (miR-196a2, miR-146a, and miR-499) with the risk of ulcerative colitis (UC) in a Japanese population.

Methods

The rs11614913 (T > C), rs2910164 (C > G), and rs3746444 (A > G) SNPs were genotyped in 170 UC and 403 control subjects.

Results

The rs3746444 AG genotype was significantly higher among the UC group (odds ratio (OR) = 1.51, 95% CI = 1.03–2.21, p = 0.037). The rs3746444 AG genotype was associated with onset at an older age (OR = 1.70, 95% CI = 1.04–2.78, p = 0.035), left-sided colitis and pancolitis (left-sided colitis, OR = 2.10, 95% CI = 1.12–3.94, p = 0.024; pancolitis, OR = 1.81, 95% CI = 1.09–3.01, p = 0.028, left-sided colitis + pancolitis, OR = 1.91, 95% CI = 1.26–2.92, p = 0.003), higher number of times hospitalized (OR = 2.63, 95% CI = 1.22–5.69, p = 0.017), steroid dependence (OR = 2.63, 95% CI = 1.27–5.44, p = 0.014), and refractory phenotypes (OR = 2.76, 95% CI = 1.46–5.21, p = 0.002) while the rs3746444 AA genotype was inversely associated with the number of times hospitalized (2∼, OR = 0.36, 95% CI = 0.17–0.79, p = 0.012), steroid dependence (OR = 0.42, 95% CI = 0.21–0.88, p = 0.021), and refractory phenotypes (OR = 0.38, 95% CI = 0.20–0.72, p = 0.003). The rs1161913 TT genotype also held a significantly higher risk of refractory phenotype (T/T vs. T/C + C/C, OR = 2.21, 95% CI = 1.17–4.18, p = 0.016).

Conclusions

Our results provided the first evidence that rs3746444 SNP may influence the susceptibility to UC, and both rs3746444 and rs11614913 SNPs may influence the pathophysiological features of UC.

Keywords

Pre-microRNAsulcerative colitispolymorphismrs11614913rs2910164rs3746444miR-196a2miR-146amiR-499

Abbreviation

UC

Ulcerative colitis

miRNA

MicroRNAs

PCR

Polymerase chain reaction

RFLP

Restricted fragment length polymorphism

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Masaaki Okubo
    • 1
  • Tomomitsu Tahara
    • 1
  • Tomoyuki Shibata
    • 1
  • Hiromi Yamashita
    • 1
  • Masakatsu Nakamura
    • 1
  • Daisuke Yoshioka
    • 1
  • Joh Yonemura
    • 1
  • Yoshio Kamiya
    • 1
  • Takamitsu Ishizuka
    • 1
  • Yoshihito Nakagawa
    • 1
  • Mitsuo Nagasaka
    • 1
  • Masami Iwata
    • 1
  • Hideto Yamada
    • 2
  • Ichiro Hirata
    • 1
  • Tomiyasu Arisawa
    • 2
  1. 1.Department of GastroenterologyFujita Health University School of MedicineAichiJapan
  2. 2.Department of GastroenterologyKanazawa Medical UniversityIshikawaJapan