IVIG Treatment and Prognosis in Guillain–Barré Syndrome
Guillain–Barré syndrome (GBS) is an acute, immune-mediated polyneuropathy that often leads to severe weakness. Intravenous immunoglobulin (IVIG) is a proven effective treatment for GBS (class 1 evidence). However, about 25% of patients need artificial ventilation and 20% are still unable to walk unaided after 6 months. Important clinical factors associated with poor outcome are age, presence of preceding diarrhea and the severity of disability in the early course of disease. These clinical factors were combined in a clinical prognostic scoring scale, the Erasmus GBS Outcome Scale (EGOS).
Materials and Methods
GBS patients being unable to walk unaided are currently treated with a standard single IVIg dose (0.4 g/kg bodyweight for 5 days). A recent retrospective study in 174 GBS patients enrolled in one of our randomized controlled clinical trials showed that patients with a minor increase of serum IgG level after standard single IVIg dose recovered significantly slower. Additionally, fewer patients reached the ability to walk unaided at six months after correction for the known clinical prognostic factors (multivariate analysis; P < 0.022).
It is yet unknown why some GBS patients only have a minor increase after standard IVIg treatment. By using the EGOS it is possible to select GBS patients with a poor prognosis. These patients potentially may benefit from a second IVIg dose.
A standard dose of IVIG is not sufficiently effective in many GBS patients. Whether these patients might benefit from a second IVIg dose needs further investigation.
Guillain–Barré syndrome (GBS) is a frequent cause of neuromuscular paralysis occurring at all ages. The incidence of GBS is reported to be 1.2–2.3 per 100,000 per year [1, 2]. GBS is a postinfectious disorder. The most frequently identified preceding infection is Campylobacter jejuni. Others are cytomegalovirus, Epstein–Barr virus, Mycoplasma pneumoniae, and Haemophilus influenzae [1, 3]. Many reports have documented the occurrence of GBS shortly after vaccinations, operations, or stressful events, but the causality and pathophysiology are still debated .
Main Features of GBS
Diagnosis of Guillain–Barré Syndrome
Features required for diagnosis
Progressive weakness in both arms and legs
Areflexia (or decreased tendon reflexes)
Features strongly supporting the diagnosis
Progression of symptoms over days to 4 weeks
Relative symmetry of symptoms
Mild sensory symptoms or signs
Cranial nerve involvement, especially bilateral weakness of facial muscles
Pain (often present)
High concentration of protein in cerebrospinal fluid (CSF)
Typical electrodiagnostic features
Features that should raise doubt about the diagnosis
Severe pulmonary dysfunction with limited limb weakness at onset
Severe sensory signs with limited weakness at onset
Bladder or bowel dysfunction at onset
Fever at onset
Sharp sensory level
Slow progression with limited weakness without respiratory involvement
(consider subacute inflammatory demyelinating polyneuropathy or CIDP)
Marked persistent asymmetry of weakness
Persistent bladder or bowel dysfunction
Increased number of mononuclear cells in CSF (>50 × 106/L)
Polymorphonuclear cells in CSF
Central nervous system involvement
Clinical Course of GBS
Molecular mimicry and cross-reactivity
C. jejuni isolates from GBS patients express lipooligosaccharides (LOS) that mimic the carbohydrates of gangliosides. The type of ganglioside mimic in C. jejuni seems to determine the specificity of the anti-ganglioside antibodies and the associated variant of GBS. Antibodies in these patients usually are cross-reactive; they recognize LOS as well as gangliosides or ganglioside complexes.
Less than 1:1,000 patients with a C. jejuni infection will develop GBS. Host factors may influence this susceptibility to develop GBS or the extent of nerve damage and outcome. Single nucleotide polymorphisms (SNPs) showed no consistent association with the susceptibility to develop GBS. Evidence indicates, however, that these SNPs may be important as disease-modifying factors. An association has been demonstrated between disease severity or outcome and SNPs in genes encoding for mannose-binding lectin, FcγRIII, MMP9, and TNF-α . However, confirmation in more extensive studies is required.
Randomized controlled trials (RCTs) have shown that PE and IVIG are effective in patients with GBS [9, 10]. Most RCTs have used the GBS disability scale to determine the effect of therapy. The GBS disability scale is a 7-point scale, ranging from no symptoms (F = 0), the ability to walk 10 m without assistance (F = 2), bedbound (F = 4), to death (F = 6). The first RCT on the use of IVIG in GBS was published in 1992 and demonstrated that IVIG is as effective as PE . IVIG is applied in a regimen of 2 g/kg bodyweight, usually as 0.4 g/kg bodyweight per day for five consecutive days. IVIG has replaced PE as the preferred treatment in many centers, mainly because of its greater convenience and availability . The combination of PE followed by IVIG was not significantly better than PE or IVIG alone . It appeared that oral steroids and, surprisingly, intravenous methylprednisolone 500 mg/day for five consecutive days alone is not beneficial in GBS . The combination of IVIG and intravenous methylprednisolone was not significantly more effective than IVIG alone. Although when a correction was made for known prognostic factors, the combined treatment shows some short-term effect . These trials show that there is an effect of immunotherapy on the course of GBS, but new studies with different regimens or another therapy that is directed toward improving the prognosis and outcome of GBS are needed.
Prognosis of GBS
The clinical course of GBS in individual patients is highly variable and difficult to predict. Advanced age is generally reported to be a negative prognostic factor. Previous studies indicate that the difference in severity of GBS can be determined in an early phase of the disease . Peroneal nerve conduction block and age above 40 years turned out to be independent predictors of disability at 6 months . We have developed a simple clinical prognostic scoring system that accurately predicts the chance of independent walking after 6 months. The Erasmus GBS Outcome Scale (EGOS) can be easily calculated after the first 2 weeks from disease onset using age, presence of preceding diarrhea, and GBS disability score . Based on the EGOS, the predicted chance to recover to independent walking after 6 months ranges from 1% to 83%. The accuracy of the scale was confirmed in an independent cohort of GBS patients. The EGOS can be used to inform individual patients about their prognosis, additionally it can be used in new treatment trials that more specifically target GBS patients with poor prognosis. A slightly modified EGOS that can calculate the prognosis only 1 week after hospital admission is currently being constructed.
Standard-Dose IVIG is not Sufficient in some GBS Patients
There is circumstantial evidence that standard IVIG dose (2 g/kg bodyweight) is not, or not sufficiently, effective in some GBS patients because a proportion of GBS patients continues to deteriorate after a standard course of IVIG . About 8% to 16% of GBS patients treated with standard-dose IVIG deteriorate again after initial improvement or stabilization. These patients have a deterioration “treatment-related fluctuation,” in which muscle force usually improves again after a repeated dose of IVIG . In addition, the results of a noncontrolled study in a small series of patients suggest that a repeated dose of IVIG may be effective in severe, unresponsive GBS patients . The results of a recent pharmacokinetic study suggest that a minor increase in serum IgG level after standard-dose IVIG is associated with a poor outcome .
Pharmacokinetics of IVIG and Outcome
In a recent study by Kuitwaard et al., serum IgG levels from 174 GBS patients were determined in samples obtained pretreatment, and at 2 weeks, 4 weeks, 3 months, and 6 months after treatment . The variation in serum IgG levels was most pronounced in the samples at 2 weeks and to a lesser extent at 4 weeks after IVIG treatment. The IgG level at 2 weeks showed a weak correlation with baseline IgG level. To investigate the pharmacokinetics of IVIG, the change in IgG was calculated by subtracting the baseline level from the level at 2 weeks (ΔIgG). There was a large variability in ΔIgG 2 weeks after IVIG treatment. It turned out that patients with a low ΔIgG (lower quartiles) had a significantly prolonged time to reach GBS disability score ≤2 (able to walk independently; log-rank P < 0.001). Using multivariate logistic regression, it was shown that baseline IgG levels or IgG levels at 2 weeks were not significantly associated with outcome. However, the increase in IgG level 2 weeks after standard IVIG (ΔIgG) was associated with outcome (P = 0.027). It appeared that patients with a ΔIgG <3.99 g/L were four times less likely (OR 0.25) and patients with a ΔIgG of 3.99 to 7.30 g/L were three times less likely (OR 0.26, 9) to walk unaided after 6 months than patients with a ΔIgG >10.92 g/L. This is the first study to show that a high increase in IgG levels after IVIG treatment for GBS is associated with a better prognosis.
IVIG is a proven effective treatment for GBS. Not all patients, however, recover enough after a standard IVIG dose. Several clinical factors are associated with the outcome. Importantly, a low increase of serum IgG levels after a standard IVIG dose appeared to be significantly associated with slower recovery and a worse prognosis. The reason why there is such a large interindividual difference in IgG increase after IVIG treatment is not yet known. The disease severity at the time of IVIG treatment appears to influence the increase in serum IgG level. The lowest increase in IgG was found in patients with more extensive disability and weakness, as defined by the GBS disability score and the Medical Research Council (MRC) sum score. It is possible that more extensive immune-mediated nerve damage results in an increase in IgG extravasation into inflamed tissues or a higher catabolic rate. These issues need to be studied further.
IVIG is an expensive treatment that may induce (generally minor) side effects and is currently not indicated (proven to be effective) in mildly affected GBS patients. A second IVIG dose potentially seems to be indicated in patients with a poor prognosis.
Factors associated with outcome after 6 months are the age of the patient, the presence of preceding diarrhea, and GBS disability score or MRC sum score 1 to 2 weeks after hospital admission; in addition, a factor to be included now seems to be the magnitude in increase of IgG levels 2 weeks after the start of IVIG [17, 19, 20]. Using these clinical factors, it is possible to select a group of GBS patients with a poor prognosis early in the course of the disease. An international trial conducted by the Inflammatory Neuropathy Consortium to study the effect of a second IVIG dose in patients with a poor prognosis based upon the EGOS is expected to start soon.
Conflict of Interest
B.C. Jacobs received an honorarium from Baxter for consultancy.
P.A. van Doorn received an unrestricted departmental research grant from Baxter to conduct a randomized controlled trial comparing Gammagard S/D with Kiovig in CIDP, and to conduct an RCT comparing Gammagard S/D with or without methylprednisolone in GBS. He also has received personal and departmental payments for consultancy/RCT board participation from Talecris Biotherapeutics, ZLB Plasma Germany, Baxter, and Octapharma AG.
The other authors have no conflicts of interest to disclose.
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