Foxp3+ Regulatory T Cells, Th17 Effector Cells, and Cytokine Environment in Inflammatory Bowel Disease
- Nicola Eastaff-LeungAffiliated withDepartment of Gastroenterology and Hepatology, The Queen Elizabeth HospitalDiscipline of Pathology, University of Adelaide
- , Nicholas MabarrackAffiliated withDiscipline of Microbiology and Immunology, University of Adelaide
- , Angela BarbourAffiliated withDiscipline of Pathology, University of Adelaide
- , Adrian CumminsAffiliated withDepartment of Gastroenterology and Hepatology, The Queen Elizabeth HospitalDiscipline of Medicine, University of Adelaide Email author
- , Simon BarryAffiliated withDiscipline of Pediatrics, University of Adelaide
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Inflammatory bowel disease (IBD) is thought to result from an aberrant immune response. Inflammation in IBD may be caused by the loss of homeostasis between CD4+ CD25high Foxp3+ regulatory cells (T reg) and proinflammatory Th17 cells. The aim of this study was to investigate T reg and Th17 cells in the peripheral blood and intestinal mucosa of IBD patients and to assess the mucosal cytokine environment.
T reg and Th17 cells were measured in peripheral blood of 63 IBD patients and 28 controls by flow cytometry. Forkhead box p3 (Foxp3), interleukin (IL)-17a, IL-1β, IL-6, IL-21, IL-23, and transforming growth factor (TGF)-β mRNA were analyzed using real-time reverse transcription polymerase chain reaction in intestinal biopsies of 24 IBD and 18 control subjects.
A decrease in T reg and increase in Th17 cells was observed in the peripheral blood of IBD patients. When measured in the same patient and expressed as a ratio, a significant decrease in T reg/Th17 ratio was observed in IBD. Elevated expression of Foxp3, IL-17a, IL-1β, and IL-6 was observed in the mucosa of IBD patients, while TGF-β was only elevated in ulcerative colitis.
IBD is associated with a reduced ratio of T reg to Th17 cells in peripheral blood and is characterized by a proinflammatory cytokine microenvironment, which supports the continued generation of Th17 cells.
KeywordsInflammatory bowel disease Crohn’s disease ulcerative colitis regulatory T cells Th17 effector cells
- Foxp3+ Regulatory T Cells, Th17 Effector Cells, and Cytokine Environment in Inflammatory Bowel Disease
Journal of Clinical Immunology
Volume 30, Issue 1 , pp 80-89
- Cover Date
- Print ISSN
- Online ISSN
- Springer US
- Additional Links
- Inflammatory bowel disease
- Crohn’s disease
- ulcerative colitis
- regulatory T cells
- Th17 effector cells
- Industry Sectors
- Author Affiliations
- 1. Department of Gastroenterology and Hepatology, The Queen Elizabeth Hospital, Woodville South, Adelaide, South Australia, 5011, Australia
- 2. Discipline of Pathology, University of Adelaide, Adelaide, South Australia, Australia
- 4. Discipline of Microbiology and Immunology, University of Adelaide, Adelaide, South Australia, Australia
- 3. Discipline of Medicine, University of Adelaide, Adelaide, South Australia, Australia
- 5. Discipline of Pediatrics, University of Adelaide, Adelaide, South Australia, Australia