Journal of Clinical Immunology

, Volume 30, Issue 1, pp 80–89

Foxp3+ Regulatory T Cells, Th17 Effector Cells, and Cytokine Environment in Inflammatory Bowel Disease

  • Nicola Eastaff-Leung
  • Nicholas Mabarrack
  • Angela Barbour
  • Adrian Cummins
  • Simon Barry
Article

DOI: 10.1007/s10875-009-9345-1

Cite this article as:
Eastaff-Leung, N., Mabarrack, N., Barbour, A. et al. J Clin Immunol (2010) 30: 80. doi:10.1007/s10875-009-9345-1

Abstract

Background

Inflammatory bowel disease (IBD) is thought to result from an aberrant immune response. Inflammation in IBD may be caused by the loss of homeostasis between CD4+ CD25high Foxp3+ regulatory cells (Treg) and proinflammatory Th17 cells. The aim of this study was to investigate Treg and Th17 cells in the peripheral blood and intestinal mucosa of IBD patients and to assess the mucosal cytokine environment.

Methods

Treg and Th17 cells were measured in peripheral blood of 63 IBD patients and 28 controls by flow cytometry. Forkhead box p3 (Foxp3), interleukin (IL)-17a, IL-1β, IL-6, IL-21, IL-23, and transforming growth factor (TGF)-β mRNA were analyzed using real-time reverse transcription polymerase chain reaction in intestinal biopsies of 24 IBD and 18 control subjects.

Results

A decrease in Treg and increase in Th17 cells was observed in the peripheral blood of IBD patients. When measured in the same patient and expressed as a ratio, a significant decrease in Treg/Th17 ratio was observed in IBD. Elevated expression of Foxp3, IL-17a, IL-1β, and IL-6 was observed in the mucosa of IBD patients, while TGF-β was only elevated in ulcerative colitis.

Conclusion

IBD is associated with a reduced ratio of Treg to Th17 cells in peripheral blood and is characterized by a proinflammatory cytokine microenvironment, which supports the continued generation of Th17 cells.

Keywords

Inflammatory bowel diseaseCrohn’s diseaseulcerative colitisregulatory T cellsTh17 effector cells

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Nicola Eastaff-Leung
    • 1
    • 2
  • Nicholas Mabarrack
    • 4
  • Angela Barbour
    • 2
  • Adrian Cummins
    • 1
    • 3
  • Simon Barry
    • 5
  1. 1.Department of Gastroenterology and HepatologyThe Queen Elizabeth HospitalAdelaideAustralia
  2. 2.Discipline of PathologyUniversity of AdelaideAdelaideAustralia
  3. 3.Discipline of MedicineUniversity of AdelaideAdelaideAustralia
  4. 4.Discipline of Microbiology and ImmunologyUniversity of AdelaideAdelaideAustralia
  5. 5.Discipline of PediatricsUniversity of AdelaideAdelaideAustralia