Journal of Clinical Immunology

, Volume 30, Issue 1, pp 60–67

Implication of Th17 and Th1 Cells in Patients with Chronic Active Hepatitis B

Authors

  • Jian Ge
    • Department of Hepatology, Qilu HospitalShandong University
    • Department of Hepatology, Qilu HospitalShandong University
  • Qing-Hua Meng
    • Department of Hepatology, Beijing YouAn HospitalCapital Medical University
  • Zhao-Xia Qi
    • Department of Hepatology, Qilu HospitalShandong University
  • Fan-Li Meng
    • Department of Hepatology, Qilu HospitalShandong University
  • Yu-Chen Fan
    • Department of Hepatology, Qilu HospitalShandong University
Article

DOI: 10.1007/s10875-009-9328-2

Cite this article as:
Ge, J., Wang, K., Meng, Q. et al. J Clin Immunol (2010) 30: 60. doi:10.1007/s10875-009-9328-2

Abstract

Objective

The pathogenesis of hepatitis B virus (HBV)-associated chronic liver disease is still not fully understood. The immune imbalance of cytokine profile exerts a profound influence on the resolution of HBV infections and HBV clearance. This present study aimed to evaluate the immune status of the peripheral T helper (Th) 17 and Th1 cells in the active patients with chronic HBV infection.

Materials and Methods

Thirty patients with chronic active hepatitis B were included in our present study. The frequency of peripheral Th 17 cells (CD3+CD8IL-17+ T cells), Th1 cells (CD3+CD8IFN-γ+ T cells), and Tc1 cells (CD3+CD8+IFN-γ+ T cells) in chronic hepatitis B (CHB) were analyzed by flow cytometry. The protein and mRNA levels of interleukin-17 (IL-17) and interferon-gamma (IFN-γ) were measured by enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction (PCR).

Results

The percentage of Th17 cells in peripheral blood of CHB patients (1.53 ± 0.52%) was significantly increased than that in normal controls (0.92 ± 0.20%; P < 0.05). In contrast, the percentage of Th1 and Tc1 cells of CHB patients was significantly decreased as compared with that of control group. The frequency of Th17 cells had a negative correlation with Th1 cells, and a positive correlation with serum alanine aminotransferase in CHB patients.

Conclusion

The elevated peripheral Th17 cells were obtained in the patient with chronic active hepatitis B, suggesting its potential role in the immune activation of chronic HBV infection.

Keywords

Chronic hepatitis BT helper 17T helper 1

Copyright information

© Springer Science+Business Media, LLC 2009