Journal of Clinical Immunology

, Volume 29, Issue 6, pp 752–760

Specific Immunotherapy to Birch Allergen Does not Enhance Suppression of Th2 Cells by CD4+CD25+ Regulatory T Cells During Pollen Season

  • Hanna Grindebacke
  • Pia Larsson
  • Kajsa Wing
  • Sabina Rak
  • Anna Rudin
Article

DOI: 10.1007/s10875-009-9312-x

Cite this article as:
Grindebacke, H., Larsson, P., Wing, K. et al. J Clin Immunol (2009) 29: 752. doi:10.1007/s10875-009-9312-x

Abstract

Introduction

The aim of this study was to investigate the suppressive capacity of CD25+ regulatory T cells on birch allergen-induced T-cell responses during the first birch pollen season after initiation of specific immunotherapy (SIT).

Methods

CD25pos and CD25neg T cells were purified from blood of birch-allergic SIT patients and birch-allergic controls, stimulated with birch pollen extract, and analyzed for T-cell proliferation and production of interferon gamma (IFN-γ), interleukin (IL)-5 and IL-10.

Results

We show that allergen-induced proliferation and IFN-γ production were suppressed equally well by CD25pos T cells from SIT patients and controls, while the IL-5 production was not suppressed by either of the groups. IL-10 levels were higher in SIT patients relative to controls only when CD25neg and CD25pos were cultured together. Furthermore, neither FOXP3 levels nor proportions of CD25high T cells were enhanced in SIT patients compared to allergic controls.

Discussion

These results suggest that the Th2-suppressive capacity of allergen-stimulated CD25pos Treg in vitro is not improved by SIT in spite of increased IL-10 production from T cells.

Keywords

HumanCD4+CD25+ regulatory T cellsallergyimmunotherapysuppressionIL-10

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Hanna Grindebacke
    • 1
  • Pia Larsson
    • 1
  • Kajsa Wing
    • 1
  • Sabina Rak
    • 2
  • Anna Rudin
    • 1
  1. 1.Department of Rheumatology and Inflammation Research, The Sahlgrenska AcademyUniversity of GothenburgGothenburgSweden
  2. 2.Department of Respiratory Medicine and Allergology, The Sahlgrenska AcademyUniversity of GothenburgGothenburgSweden