Date: 20 Jun 2009
Specific Immunotherapy to Birch Allergen Does not Enhance Suppression of Th2 Cells by CD4+CD25+ Regulatory T Cells During Pollen Season
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The aim of this study was to investigate the suppressive capacity of CD25+ regulatory T cells on birch allergen-induced T-cell responses during the first birch pollen season after initiation of specific immunotherapy (SIT).
CD25pos and CD25neg T cells were purified from blood of birch-allergic SIT patients and birch-allergic controls, stimulated with birch pollen extract, and analyzed for T-cell proliferation and production of interferon gamma (IFN-γ), interleukin (IL)-5 and IL-10.
We show that allergen-induced proliferation and IFN-γ production were suppressed equally well by CD25pos T cells from SIT patients and controls, while the IL-5 production was not suppressed by either of the groups. IL-10 levels were higher in SIT patients relative to controls only when CD25neg and CD25pos were cultured together. Furthermore, neither FOXP3 levels nor proportions of CD25high T cells were enhanced in SIT patients compared to allergic controls.
These results suggest that the Th2-suppressive capacity of allergen-stimulated CD25pos Treg in vitro is not improved by SIT in spite of increased IL-10 production from T cells.
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- Specific Immunotherapy to Birch Allergen Does not Enhance Suppression of Th2 Cells by CD4+CD25+ Regulatory T Cells During Pollen Season
Journal of Clinical Immunology
Volume 29, Issue 6 , pp 752-760
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- Springer US
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- CD4+CD25+ regulatory T cells
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- Author Affiliations
- 1. Department of Rheumatology and Inflammation Research, The Sahlgrenska Academy, University of Gothenburg, Guldhedsgatan 10, 413 46, Gothenburg, Sweden
- 2. Department of Respiratory Medicine and Allergology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden