Journal of Clinical Immunology

, Volume 29, Issue 5, pp 665–673

Clinico-immunologic Study on Immunotherapy with Mixed and Single Insect Allergens

  • Deepsikha Srivastava
  • Bhanu P. Singh
  • Naveen Arora
  • Shailendra Nath Gaur

DOI: 10.1007/s10875-009-9307-7

Cite this article as:
Srivastava, D., Singh, B.P., Arora, N. et al. J Clin Immunol (2009) 29: 665. doi:10.1007/s10875-009-9307-7



Immunotherapy (IT) is practiced mainly with mixed and single allergen vaccines. But studies are rare with mixed allergen preparations.


The objective of this study is to study mix and single insect allergen IT in patients of allergic rhinitis and asthma.


We performed a double-blind placebo-controlled trial of mix and single allergen IT for 1 year in 99 patients of asthma or rhinitis or both. There were two groups, (1) active allergen IT (n = 61) with three subgroups single insect extract (cockroach, housefly, or mosquito) and mix allergen IT (two or three insect extracts) and (2) placebo (n = 38). Clinical (skin reactivity, airway reactivity, and symptom score) and immunological (IgE/IgG4 and IgG1/IgG4 ratio) parameters were assessed at baseline and after 1 year of IT.


Eighty-five patients completed 1 year of IT. The active allergen IT group patients showed a significant improvement compared to baseline values (p < 0.05) and placebo group patients (p < 0.05) with regard to symptom scores, FEV1 values, and immunological parameters (IgG4). No significant difference was found between mixed and single IT group patients for changes in clinical and immunological parameters. Positive correlation was observed between increase in IgG4 and clinical improvement. The changes in above parameters in placebo group were nonsignificant after 1 year of treatment.


IT with two to three mix extract from the same allergen group is effective for insect hypersensitivity.


Insect allergyimmunotherapydouble-blind placebo-controlled trialpulmonary function testELISA

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Deepsikha Srivastava
    • 1
    • 2
  • Bhanu P. Singh
    • 1
  • Naveen Arora
    • 1
  • Shailendra Nath Gaur
    • 3
  1. 1.Institute of Genomics and Integrative BiologyDelhiIndia
  2. 2.University of PunePuneIndia
  3. 3.Department of Pulmonary Medicine, Vallabhbhai Patel Chest InstituteUniversity of DelhiDelhiIndia