Journal of Clinical Immunology

, Volume 28, Issue 5, pp 486–494

Elevated Serum Anti-Saccharomyces cerevisiae, Anti-I2 and Anti-OmpW Antibody Levels in Patients with Suspicion of Celiac Disease

  • Sara Ashorn
  • Hanna Raukola
  • Tuuli Välineva
  • Merja Ashorn
  • Bo Wei
  • Jonathan Braun
  • Immo Rantala
  • Katri Kaukinen
  • Tiina Luukkaala
  • Pekka Collin
  • Markku Mäki
  • Sari Iltanen
Article

DOI: 10.1007/s10875-008-9200-9

Cite this article as:
Ashorn, S., Raukola, H., Välineva, T. et al. J Clin Immunol (2008) 28: 486. doi:10.1007/s10875-008-9200-9

Abstract

Objectives

Expression of anti-Saccharomyces cerevisiae antibodies (ASCA) identifies patients and individuals at risk for Crohn’s disease and has also been reported in 40–60% of celiac disease (CD) cases, suggesting a role of host response to enteric microbiota in the development of inflammatory lesions. In this prospective study in patients with suspicion of CD, we evaluate the frequency and association of ASCA to serological responses for other host microbial targets formally associated with Crohn’s disease, including the Pseudomonas fluorescens associated sequence I2 and a Bacteroides caccae TonB-linked outer membrane protein, OmpW.

Methods

Small bowel mucosal biopsies were taken from 242 patients with suspicion of CD, their sera were tested for antibodies to tissue transglutaminase (tTG), ASCA, I2, and OmpW. Eighty adult healthy blood donors were used as controls.

Results

The diagnosis of CD was confirmed on biopsy in 134 cases. The occurrence of ASCA and I2 positivity was significantly higher in adult CD patients than in patients with non-CD disease. Anti-I2 levels in the sera were significantly higher in adult CD patients than in non-CD disease or the controls and anti-OmpW levels in CD and non-CD patients when compared to controls. Positive seroreactivity to OmpW seemed to increase with age. Of the CD patients, 90% were seropositive for at least one microbial antigen tested.

Conclusions

This study demonstrates a mosaic of disease-related serological responses to microbial antigens in patients with CD. Immune responses to commensal enteric bacteria may play a role in the small intestine mucosal damage in CD.

Keywords

Tissue transglutaminaseI2OmpWASCAserologyceliac disease

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Sara Ashorn
    • 1
  • Hanna Raukola
    • 3
  • Tuuli Välineva
    • 6
  • Merja Ashorn
    • 1
    • 2
  • Bo Wei
    • 8
  • Jonathan Braun
    • 8
  • Immo Rantala
    • 4
  • Katri Kaukinen
    • 1
    • 5
  • Tiina Luukkaala
    • 7
  • Pekka Collin
    • 5
  • Markku Mäki
    • 1
    • 2
  • Sari Iltanen
    • 1
    • 9
  1. 1.Paediatric Research Centre and Medical SchoolUniversity of Tampere and Tampere University HospitalTampereFinland
  2. 2.Department of PaediatricsTampere University HospitalTampereFinland
  3. 3.Department of MicrobiologyTampere University HospitalTampereFinland
  4. 4.Department of PathologyTampere University HospitalTampereFinland
  5. 5.Department of Gastroenterology and Alimentary Tract SurgeryTampere University HospitalTampereFinland
  6. 6.Institute of Medical TechnologyUniversity of TampereTampereFinland
  7. 7.Research Unit, Tampere University Hospital and Tampere School of Public HealthUniversity of TampereTampereFinland
  8. 8.Department of Pathology and Laboratory MedicineUniversity of CaliforniaLos AngelesUSA
  9. 9.Paediatric Research CentreUniversity of TampereTampereFinland