Journal of Clinical Immunology

, Volume 28, Issue 3, pp 220–231

Interferon-α Induces Up-regulation and Nuclear Translocation of the Ro52 Autoantigen as Detected by a Panel of Novel Ro52-specific Monoclonal Antibodies

  • Linn Strandberg
  • Aurelie Ambrosi
  • Alexander Espinosa
  • Lars Ottosson
  • Maija-Leena Eloranta
  • Wei Zhou
  • Åse Elfving
  • Edward Greenfield
  • Vijay K. Kuchroo
  • Marie Wahren-Herlenius
Article

DOI: 10.1007/s10875-007-9157-0

Cite this article as:
Strandberg, L., Ambrosi, A., Espinosa, A. et al. J Clin Immunol (2008) 28: 220. doi:10.1007/s10875-007-9157-0

Abstract

Interferon-α (IFN-α) has been implicated in the pathogenesis of Sjögren’s syndrome and systemic lupus erythematosus. Ro52, which was recently identified as an E3 ligase with anti-proliferative and pro-apoptotic properties, is a major autoantigen targeted in both these conditions. Microarray analyses have indicated up-regulation of Ro52 by INF-α, and the objective of the present study was to address the potential link between IFN-α and Ro52. To investigate the influence of IFN-α on Ro52 protein levels and cellular localization, we generated a panel of monoclonal antibodies to different domains of Ro52. These novel monoclonal antibodies were characterized by immunoprecipitation, Western blot, and enzyme-linked immunosorbent assay using cell lysates, recombinant Ro52 protein, and synthetic peptides. Ro52 was up-regulated in HeLa cells and human B cells at the messenger RNA and protein levels in response to IFN-α stimulation as detected by reverse transcriptase polymerase chain reaction and Western blot. After up-regulation, Ro52 translocated from the cytoplasm to the nucleus. The nuclear translocation of Ro52 was observed after staining with generated monoclonal antibodies specific for both the RING, coiled-coil, and B30.2 domains of Ro52 and the nuclear translocation of Ro52 preceded IFN-α-induced apoptotic cell death detected by caspase-3 and TUNEL staining in the treated cultures. In conclusion, our data show that IFN-α first induces up-regulation of Ro52 protein and then prompts translocation of the up-regulated Ro52 protein in to the nucleus. The translocation precedes apoptosis of the IFN-α exposed cells, suggesting a role for Ro52 in mediating the anti-proliferative or pro-apoptotic effects of the autoimmune-related cytokine IFN-α.

Keywords

Ro52 interferon-α E3 ligase Sjögren’s syndrome SLE 

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Linn Strandberg
    • 1
  • Aurelie Ambrosi
    • 1
  • Alexander Espinosa
    • 1
  • Lars Ottosson
    • 1
  • Maija-Leena Eloranta
    • 2
  • Wei Zhou
    • 1
  • Åse Elfving
    • 1
  • Edward Greenfield
    • 3
  • Vijay K. Kuchroo
    • 3
  • Marie Wahren-Herlenius
    • 1
  1. 1.Rheumatology Unit, Department of Medicine, CMM L8:04Karolinska InstitutetStockholmSweden
  2. 2.Department of Medical SciencesUppsala University HospitalUppsalaSweden
  3. 3.Center for Neurologic Diseases, Brigham and Woman’s HospitalHarvard Medical SchoolBostonUSA

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