Journal of Clinical Immunology

, Volume 27, Issue 4, pp 409–429

Involvement of MCP-1 in Tubulointerstitial Fibrosis Through Massive Proteinuria in Anti-GBM Nephritis Induced in WKY Rats

  • Hajime Taniguchi
  • Ryoji Kojima
  • Hiroko Sade
  • Mayumi Furuya
  • Norio Inomata
  • Mikio Ito
Original Paper

DOI: 10.1007/s10875-007-9085-z

Cite this article as:
Taniguchi, H., Kojima, R., Sade, H. et al. J Clin Immunol (2007) 27: 409. doi:10.1007/s10875-007-9085-z

We investigated participation of monocyte chemoattractant protein-1 (MCP-1) in tubulointerstitial fibrosis and correlation between MCP-1 and proteinuria in Wistar-Kyoto (WKY) rats with glomerulonephritis induced by anti-glomerular basement membrane (anti-GBM) antibody. WKY rats showed marked proteinuria and severe glomerular crescent formation at 7 days post antibody injection. At 28 days, tubulointerstitial fibrotic lesions were observed, followed by sustained heavy proteinuria and severe tubulointerstitial fibrosis at 56 days. Histological examination revealed that the overlapped immunoreactivities of MCP-1, rat albumin, and p65NF-κB were detected in the same tubular segments of nephritic kidney, and a significant positive correlation was observed between proteinuria and MCP-1 expression in the tubulointerstitial fibrosis. ED-1- and CD8-positive cells were also abundant, and there was a good correlation between monocyte/macrophage recruitment and MCP-1 expression in the tubulointerstitial area. These results suggest that MCP-1 participates in the progression of tubulointerstitial fibrosis, through massive albuminuria, which is accompanied by marked monocyte/macrophage recruitment.

KEY WORDS:

Monocyte chemoattractant protein-1 (MCP-1) Proteinuria Tubulointerstitial fibrosis Wistar-Kyoto (WKY) Anti-glomerular basement membrane (anti-GBM) 

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Hajime Taniguchi
    • 1
  • Ryoji Kojima
    • 2
    • 3
  • Hiroko Sade
    • 1
  • Mayumi Furuya
    • 1
  • Norio Inomata
    • 1
  • Mikio Ito
    • 2
  1. 1.Biomedical Research LaboratoriesDaiichi Asubio Pharma Co., Ltd.OsakaJapan
  2. 2.Laboratory of Analytical PharmacologyFaculty of Pharmacy, Meijo UniversityNagoyaJapan
  3. 3.Ryoji Kojima Laboratory of Analytical PharmacologyFaculty of Pharmacy, Meijo UniversityNagoyaJapan