Original Paper

Journal of Clinical Immunology

, Volume 27, Issue 3, pp 317-326

First online:

CD4 +CD25 + Regulatory T Cells Decreased the Antitumor Activity of Cytokine-Induced Killer (CIK) Cells of Lung Cancer Patients

  • Hui LiAffiliated withDepartment of Immunology, Cancer Institute and Hospital, Tianjin Medical University
  • , Jin-Pu YuAffiliated withDepartment of Immunology, Cancer Institute and Hospital, Tianjin Medical University
  • , Shui CaoAffiliated withDepartment of Immunology, Cancer Institute and Hospital, Tianjin Medical University
  • , Feng WeiAffiliated withDepartment of Immunology, Cancer Institute and Hospital, Tianjin Medical University
  • , Peng ZhangAffiliated withDepartment of Immunology, Cancer Institute and Hospital, Tianjin Medical University
  • , Xiu-Mei AnAffiliated withDepartment of Immunology, Cancer Institute and Hospital, Tianjin Medical University
  • , Zong-Tang HuangAffiliated withDepartment of Immunology, Cancer Institute and Hospital, Tianjin Medical University
  • , Xiu-Bao RenAffiliated withDepartment of Immunology, Cancer Institute and Hospital, Tianjin Medical University Email author 

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CD4 +CD25 + regulatory T cells (Tregs) have been shown to inhibit cytotoxic lymphocytes-mediated immune responses. Cytokine-induced killer (CIK) cells exert high impact on adoptive immunotherapeutic approaches. Therefore, the purpose of this report was to determine the effect of Tregs on CIK cell growth and CIK-induced cytotoxicity for inhibition of tumor growth in vivo as well as in vitro. After depletion of CD4 +CD25 + cells before culture, the proliferation and cytotoxicity of CIK cells, which indicated in bromodeoxyuridine (BrdU) and lactic dehydrogenase (LDH) assays, were significantly increased. Depletion of CD4 +CD25 + cells preculture also enhanced the suppression effect on the lung cancer cells inoculated in experimental animals. Blockage of glucocorticoid-induced tumor necrosis factor receptor (GITR) and transforming growth factor β1 (TGF-β1) by antibodies partially abrogated the suppressive effect of CD4 +CD25 + cells on CIK. These results indicated that Tregs could inhibit the antitumor activity of CIK cells. The molecules TGF-β and GITR may contribute to the suppressive function of CD4 +CD25 + cells.

KEY WORDS:

CD4 +CD25 + regulatory T cells CIK GITR TGF-β