Chemotherapeutic agent is characterized by its concentration in tumor cells with minimum side effects. Oleandrin, a polyphenolic cardiac glycoside is known to induce apoptosis in tumor cells. However, no report is available on its efficacy in primary cells. In this report we are providing the evidence that oleandrin induces apoptosis, not necrosis in tumor cells but not in primary cells like peripheral blood mononuclear cells (PBMC) and neutrophils. Oleandrin inhibited NF-κB activation in tumor cells but not in primary cells. It induced cell death in NF-κB-overexpressed tumor cells. Oleandrin induced Fas expression thereby inducing apoptosis in tumor cells but not in primary cells. Dominant negative FADD inhibited oleandrin-induced cell death in tumor cells. Overall, these results suggest that oleandrin mediates apoptosis in tumor cells by inducing Fas but not in primary cells indicating its potential anti-cancer property with no or slight side effect.
Abbreviations
- FADD:
-
Fas-associated death domain
- GFP:
-
green fluorescent peptide
- IκBα:
-
inhibitory subunit of NF-κB
- IκBα-DN:
-
IκBα dominant negative
- LDH:
-
lactate dehydrogenase
- MDA:
-
malondialdehyde
- NF-κB:
-
nuclear transcription factor kappa B
- MTT:
-
3-(4,5-dimethyl-2-thiozolyl)-2,5-diphenyl-2H-tetrazolium bromide
- PARP:
-
poly-ADP ribose polymerase
- PBMC:
-
peripheral blood mononuclear cells
- PIS:
-
preimmune serum
- ROI:
-
reactive oxygen intermediates
- SA-LPS:
-
serum-activated lipopolysaccharide
- SEAP:
-
secretory alkaline phosphatase.
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ACKNOWLEDGMENTS
The Department of Biotechnology (DBT), Government of India supported this work. We duly acknowledge Department of Science and Technology (DST) for providing SEARC Fast Track proposal for Young Scientists grant to SM, and Council for Scientific and Industrial Research (CSIR), New Delhi for providing fellowship for YS.
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A retraction note to this article is available at http://dx.doi.org/10.1007/s10875-013-9884-3.
This article has been retracted at the request of the Centre for DNA Fingerprinting and Diagnostics as it contains manipulated figures.
An erratum to this article is available at http://dx.doi.org/10.1007/s10875-013-9884-3.
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SREENIVASAN, Y., RAGHAVENDRA, P.B. & MANNA, S.K. RETRACTED ARTICLE: Oleandrin-Mediated Expression of Fas Potentiates Apoptosis in Tumor Cells. J Clin Immunol 26, 308–322 (2006). https://doi.org/10.1007/s10875-006-9028-0
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DOI: https://doi.org/10.1007/s10875-006-9028-0