Journal of Oceanography

, Volume 69, Issue 5, pp 591–600

Detection of ammonia-oxidizing Bacteria and Archaea within coral reef cyanobacterial mats

Authors

    • School of Arts and SciencesUniversity of Houston-Victoria
  • Matthew R. Boyett
    • School of Arts and SciencesUniversity of Houston-Victoria
  • Edwin Cruz-Rivera
    • Asian University for Women
Original Article

DOI: 10.1007/s10872-013-0195-3

Cite this article as:
Sobolev, D., Boyett, M.R. & Cruz-Rivera, E. J Oceanogr (2013) 69: 591. doi:10.1007/s10872-013-0195-3

Abstract

Coral-reef filamentous cyanobacterial mats are complex communities of microbes. We used DGGE and eubacterial and archaeal 16S rRNA and amoA ammonium monooxygenase subunit A primer sets to explore the presence of genes mediating nitrogen conversion within cyanobacterial mats from Hawaii. DGGE band analysis revealed the presence of halophiles such as Halococcus salifodinae and of Nitrosopumilus-like organisms. Twenty seven out of 31 clone sequences exhibited a 95 % or greater 16S rRNA gene identity with known archaeal ammonia oxidizers such as Nitrosopumilus maritimus. The presence of Archaea within those mats, as well as the co-occurrence of both ammonia-oxidizing Archaea and ammonia-oxidizing Bacteria suggests importance of the former in the cyanobacterial mat community, and suggests greater than anticipated diversity of nitrogen conversion processes and organisms in those systems.

Keywords

BacteriaArchaeaCoral reefNitrogen cycleCyanobacterial matLyngbyaNitrosopumilus16SamoA

Copyright information

© The Oceanographic Society of Japan and Springer Japan 2013