Journal of Biological Physics

, Volume 32, Issue 1, pp SN1–SN4

Single-Fluorophore Diffusion in a Lipid Membrane over a Subwavelength Aperture

  • Jérôme Wenger
  • Hervé Rigneault
  • José Dintinger
  • Didier Marguet
  • Pierre-François Lenne
Short Note

DOI: 10.1007/s10867-006-2909-x

Cite this article as:
Wenger, J., Rigneault, H., Dintinger, J. et al. J Biol Phys (2006) 32: SN1. doi:10.1007/s10867-006-2909-x

Abstract

We use submicrometer apertures milled in an aluminium film to study the diffusion dynamics of β-Bodipy-FL-C5-HPC (Bodipy-PC) fluorophores in a lipid dioleoylphosphatidylcholine (DOPC) multilayer. The observation volume is limited by the aperture diameter, well below the optical wavelength. This spatial resolution improvement comes together with an enhancement of the detected fluorescence per molecule as compared to an open sample, with a significant increase up to 3.5 times.

Key words

fluorescence correlation spectroscopyconfocal microscopynano-apertureslipid bilayer

Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • Jérôme Wenger
    • 1
  • Hervé Rigneault
    • 1
  • José Dintinger
    • 2
  • Didier Marguet
    • 3
  • Pierre-François Lenne
    • 1
  1. 1.Domaine Universitaire de Saint JérômeInstitut Fresnel, Université Paul Cézanne Aix-Marseille IIIFrance
  2. 2.ISISUniversité Louis PasteurStrasbourgFrance
  3. 3.Centre d'Immunologie de Marseille-LuminyCNRS UMR 6102 INSERM UMRMarseilleFrance