Journal of Biological Physics

, Volume 32, Issue 1, pp SN1–SN4

Single-Fluorophore Diffusion in a Lipid Membrane over a Subwavelength Aperture

Authors

  • Jérôme Wenger
    • Domaine Universitaire de Saint JérômeInstitut Fresnel, Université Paul Cézanne Aix-Marseille III
  • Hervé Rigneault
    • Domaine Universitaire de Saint JérômeInstitut Fresnel, Université Paul Cézanne Aix-Marseille III
  • José Dintinger
    • ISISUniversité Louis Pasteur
  • Didier Marguet
    • Centre d'Immunologie de Marseille-LuminyCNRS UMR 6102 INSERM UMR
    • Domaine Universitaire de Saint JérômeInstitut Fresnel, Université Paul Cézanne Aix-Marseille III
Short Note

DOI: 10.1007/s10867-006-2909-x

Cite this article as:
Wenger, J., Rigneault, H., Dintinger, J. et al. J Biol Phys (2006) 32: SN1. doi:10.1007/s10867-006-2909-x

Abstract

We use submicrometer apertures milled in an aluminium film to study the diffusion dynamics of β-Bodipy-FL-C5-HPC (Bodipy-PC) fluorophores in a lipid dioleoylphosphatidylcholine (DOPC) multilayer. The observation volume is limited by the aperture diameter, well below the optical wavelength. This spatial resolution improvement comes together with an enhancement of the detected fluorescence per molecule as compared to an open sample, with a significant increase up to 3.5 times.

Key words

fluorescence correlation spectroscopyconfocal microscopynano-apertureslipid bilayer

Copyright information

© Springer Science + Business Media, Inc. 2006