Short Note

Journal of Biological Physics

, Volume 32, Issue 1, pp SN1-SN4

First online:

Single-Fluorophore Diffusion in a Lipid Membrane over a Subwavelength Aperture

  • Jérôme WengerAffiliated withDomaine Universitaire de Saint Jérôme, Institut Fresnel, Université Paul Cézanne Aix-Marseille III
  • , Hervé RigneaultAffiliated withDomaine Universitaire de Saint Jérôme, Institut Fresnel, Université Paul Cézanne Aix-Marseille III
  • , José DintingerAffiliated withISIS, Université Louis Pasteur
  • , Didier MarguetAffiliated withCentre d'Immunologie de Marseille-Luminy, CNRS UMR 6102 INSERM UMR
  • , Pierre-François LenneAffiliated withDomaine Universitaire de Saint Jérôme, Institut Fresnel, Université Paul Cézanne Aix-Marseille III Email author 

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Abstract

We use submicrometer apertures milled in an aluminium film to study the diffusion dynamics of β-Bodipy-FL-C5-HPC (Bodipy-PC) fluorophores in a lipid dioleoylphosphatidylcholine (DOPC) multilayer. The observation volume is limited by the aperture diameter, well below the optical wavelength. This spatial resolution improvement comes together with an enhancement of the detected fluorescence per molecule as compared to an open sample, with a significant increase up to 3.5 times.

Key words

fluorescence correlation spectroscopy confocal microscopy nano-apertures lipid bilayer