Journal of Bioenergetics and Biomembranes

, Volume 44, Issue 1, pp 155–161

Transport and cytotoxicity of the anticancer drug 3-bromopyruvate in the yeast Saccharomyces cerevisiae

  • Paweł Lis
  • Marek Zarzycki
  • Young H. Ko
  • Margarida Casal
  • Peter L. Pedersen
  • Andre Goffeau
  • Stanisław Ułaszewski
Open AccessArticle

DOI: 10.1007/s10863-012-9421-8

Cite this article as:
Lis, P., Zarzycki, M., Ko, Y.H. et al. J Bioenerg Biomembr (2012) 44: 155. doi:10.1007/s10863-012-9421-8

Abstract

We have investigated the cytotoxicity in Saccharomyces cerevisiae of the novel antitumor agent 3-bromopyruvate (3-BP). 3-BP enters the yeast cells through the lactate/pyruvate H+ symporter Jen1p and inhibits cell growth at minimal inhibitory concentration of 1.8 mM when grown on non-glucose conditions. It is not submitted to the efflux pumps conferring Pleiotropic Drug Resistance in yeast. Yeast growth is more sensitive to 3-BP than Gleevec (Imatinib methanesulfonate) which in contrast to 3-BP is submitted to the PDR network of efflux pumps. The sensitivity of yeast to 3-BP is increased considerably by mutations or chemical treatment by buthionine sulfoximine that decrease the intracellular concentration of glutathione.

Keywords

Saccharomyces cerevisiae3-bromopyruvateMultidrug resistanceTransportGlutathioneGleevecAnti-cancer chemotherapy
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Copyright information

© The Author(s) 2012

Authors and Affiliations

  • Paweł Lis
    • 1
  • Marek Zarzycki
    • 1
  • Young H. Ko
    • 2
  • Margarida Casal
    • 3
  • Peter L. Pedersen
    • 4
  • Andre Goffeau
    • 5
  • Stanisław Ułaszewski
    • 1
  1. 1.Institute of Genetics and MicrobiologyUniversity of WroclawWroclawPoland
  2. 2.Owings MillsUSA
  3. 3.Department of Biology, Molecular and Environmental Biology Centre (CBMA)University of MinhoBragaPortugal
  4. 4.Department of Biological ChemistryJohn Hopkins University School of MedicineBaltimoreUSA
  5. 5.Institut des Sciences de la Vie, Université Catholique de Louvain-la-Neuve, 1, Place de l’UniversitéLouvain-la-NeuveBelgium