Journal of Bioenergetics and Biomembranes

, 43:483

Respiratory and TCA cycle activities affect S. cerevisiae lifespan, response to caloric restriction and mtDNA stability

Authors

  • Erich B. Tahara
    • Departamento de Bioquímica, Instituto de QuímicaUniversidade de São Paulo
  • Kizzy Cezário
    • Departamento de Microbiologia, Instituto de Ciências BiomédicasUniversidade de São Paulo
  • Nadja C. Souza-Pinto
    • Departamento de Bioquímica, Instituto de QuímicaUniversidade de São Paulo
  • Mario H. Barros
    • Departamento de Microbiologia, Instituto de Ciências BiomédicasUniversidade de São Paulo
    • Departamento de Bioquímica, Instituto de QuímicaUniversidade de São Paulo
Article

DOI: 10.1007/s10863-011-9377-0

Cite this article as:
Tahara, E.B., Cezário, K., Souza-Pinto, N.C. et al. J Bioenerg Biomembr (2011) 43: 483. doi:10.1007/s10863-011-9377-0
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Abstract

We studied the importance of respiratory fitness in S. cerevisiae lifespan, response to caloric restriction (CR) and mtDNA stability. Mutants harboring mtDNA instability and electron transport defects do not respond to CR, while tricarboxylic acid cycle mutants presented extended lifespans due to CR. Interestingly, mtDNA is unstable in cells lacking dihydrolipoyl dehydrogenase under CR conditions, and cells lacking aconitase under standard conditions (both enzymes are components of the TCA and mitochondrial nucleoid). Altogether, our data indicate that respiratory integrity is required for lifespan extension by CR and that mtDNA stability is regulated by nucleoid proteins in a glucose-sensitive manner.

Keywords

AgingCalorie restrictionMitochondriaRespirationYeastKrebs cycle
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Copyright information

© Springer Science+Business Media, LLC 2011