Journal of Bioenergetics and Biomembranes

, Volume 43, Issue 2, pp 175–180

Accumulation of dodecyltriphenylphosphonium in mitochondria induces their swelling and ROS-dependent growth inhibition in yeast

Authors

  • Silvia M. Ojovan
    • Faculty of Bioengineering and BioinformaticsMoscow State University
  • Dmitry A. Knorre
    • Belozersky Institute of Physico-Chemical Biology and Institute of MitoengineeringMoscow State University
  • Olga V. Markova
    • Belozersky Institute of Physico-Chemical Biology and Institute of MitoengineeringMoscow State University
  • Ekaterina A. Smirnova
    • Belozersky Institute of Physico-Chemical Biology and Institute of MitoengineeringMoscow State University
  • Lora E. Bakeeva
    • Belozersky Institute of Physico-Chemical Biology and Institute of MitoengineeringMoscow State University
    • Belozersky Institute of Physico-Chemical Biology and Institute of MitoengineeringMoscow State University
    • Belozersky Institute of Physico-Chemical BiologyMoscow State University
Article

DOI: 10.1007/s10863-011-9345-8

Cite this article as:
Ojovan, S.M., Knorre, D.A., Markova, O.V. et al. J Bioenerg Biomembr (2011) 43: 175. doi:10.1007/s10863-011-9345-8

Abstract

Hydrophobic cations with delocalized charge are used to deliver drugs to mitochondria. However, micromolar concentrations of such compounds could be toxic due to their excessive accumulation in mitochondria. We studied possible pathophysiological effects of one such cation, i.e. dodecyltriphenylphosphonium (C12-TPP), in the yeast Saccharomyces cerevisiae. First, we found that C12-TPP induces high-amplitude mitochondrial swelling. The swelling can be prevented by addition of protonophorous uncoupler FCCP or antioxidant alpha-tocopherol, but not other tested antioxidants (N-acetylcysteine and Trolox). Second, FCCP prevents ROS-sensitive fluorescent dye (dichlorofluorescein diacetate) staining of yeast treated with C12-TPP. We also showed that all tested antioxidants partially restore the growth inhibited by C12-TPP. The latter points that ROS rather than the mitochondria swelling limit the growth rate.

Keywords

YeastPenetrating cationsDodecyltriphenylphosphoniumMitochondriaSwellingROS

Copyright information

© Springer Science+Business Media, LLC 2011