Article

Journal of Bioenergetics and Biomembranes

, Volume 40, Issue 5, pp 419-424

Open Access This content is freely available online to anyone, anywhere at any time.

The higher level of organization of the oxidative phosphorylation system: mitochondrial supercomplexes

  • Natalya V. DudkinaAffiliated withElectron microscopy group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen
  • , Stephanie SunderhausAffiliated withInstitute for Plant Genetics, Faculty of Natural Sciences, Leibniz Universität Hannover
  • , Egbert J. BoekemaAffiliated withElectron microscopy group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Email author 
  • , Hans-Peter BraunAffiliated withInstitute for Plant Genetics, Faculty of Natural Sciences, Leibniz Universität Hannover

Abstract

The organization of the oxidative phosphorylation (OXPHOS) system within the inner mitochondrial membrane appears to be far more complicated than previously thought. In particular, the individual protein complexes of the OXPHOS system (complexes I to V) were found to specifically interact forming defined supramolecular structures. Blue-native polyacrylamide gel electrophoresis and single particle electron microscopy proved to be especially valuable in studying the so-called “respiratory supercomplexes”? Based on these procedures, increasing evidence was presented supporting a “solid state” organization of the OXPHOS system. Here, we summarize results on the formation, organisation and function of the various types of mitochondrial OXPHOS supercomplexes.

Keywords

Supercomplexes Respirasome Electron microscopy Mitochondria Oxidative phosphorylation