Journal of Bioenergetics and Biomembranes

, Volume 37, Issue 5, pp 307–317

ANT2 Isoform Required for Cancer Cell Glycolysis

  • Arnaud Chevrollier
  • Dominique Loiseau
  • Béatrice Chabi
  • Gilles Renier
  • Olivier Douay
  • Yves Malthièry
  • Georges Stepien
Article

DOI: 10.1007/s10863-005-8642-5

Cite this article as:
Chevrollier, A., Loiseau, D., Chabi, B. et al. J Bioenerg Biomembr (2005) 37: 307. doi:10.1007/s10863-005-8642-5

Abstract

The three adenine nucleotide translocator ({ANT1} to {ANT3}) isoforms, differentially expressed in human cells, play a crucial role in cell bioenergetics by catalyzing ADP and ATP exchange across the mitochondrial inner membrane. In contrast to differentiated tissue cells, transformed cells, and their ρ0 derivatives, i.e. cells deprived of mitochondrial DNA, sustain a high rate of glycolysis. We compared the expression pattern of {ANT} isoforms in several transformed human cell lines at different stages of the cell cycle. The level of {ANT2} expression and glycolytic ATP production in these cell lines were in keeping with their metabolic background and their state of differentiation. The sensitivity of the mitochondrial inner membrane potential (Δψ) to several inhibitors of glycolysis and oxidative phosphorylation confirmed this relationship. We propose a new model for ATP uptake in cancer cells implicating the {ANT2} isoform, in conjunction with hexokinase II and the β subunit of mitochondrial ATP synthase, in the Δψ maintenance and in the aggressiveness of cancer cells.

Keywords

Carcinogenesismitochondriaglycolysisadenine nucleotide translocator.

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Arnaud Chevrollier
    • 1
    • 2
    • 3
  • Dominique Loiseau
    • 1
    • 2
    • 3
  • Béatrice Chabi
    • 4
    • 5
  • Gilles Renier
    • 1
    • 2
    • 3
  • Olivier Douay
    • 1
    • 2
    • 3
  • Yves Malthièry
    • 1
    • 2
    • 3
  • Georges Stepien
    • 5
    • 6
  1. 1.InsermAngersFrance
  2. 2.Univ AngersAngersFrance
  3. 3.CHRU Angers, Service d' ImmunologieAngersFrance
  4. 4.INRAFrance
  5. 5.Univ AuvergneFrance
  6. 6.InsermFrance