Journal of Biomolecular NMR

, Volume 56, Issue 4, pp 359–363

Cryogenic solid state NMR studies of fibrils of the Alzheimer’s disease amyloid-β peptide: perspectives for DNP

  • Juan-Miguel Lopez del Amo
  • Dennis Schneider
  • Antoine Loquet
  • Adam Lange
  • Bernd Reif
Article

DOI: 10.1007/s10858-013-9755-5

Cite this article as:
Lopez del Amo, J., Schneider, D., Loquet, A. et al. J Biomol NMR (2013) 56: 359. doi:10.1007/s10858-013-9755-5

Abstract

Dynamic Nuclear Polarization solid-state NMR holds the potential to enable a dramatic increase in sensitivity by exploiting the large magnetic moment of the electron. However, applications to biological solids are hampered in uniformly isotopically enriched biomacromolecules due to line broadening which yields a limited spectral resolution at cryogenic temperatures. We show here that high magnetic fields allow to overcome the broadening of resonance lines often experienced at liquid nitrogen temperatures. For a fibril sample of the Alzheimer’s disease β-amyloid peptide, we find similar line widths at low temperature and at room temperature. The presented results open new perspectives for structural investigations in the solid-state.

Keywords

Dynamic nuclear polarization (DNP)Magic angle spinning (MAS)Solid-stateNMR high magnetic fieldsAlzheimer’s β-amyloid fibrils

Supplementary material

10858_2013_9755_MOESM1_ESM.docx (786 kb)
Supplementary material 1 (DOCX 785 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Juan-Miguel Lopez del Amo
    • 1
    • 2
    • 3
    • 6
  • Dennis Schneider
    • 4
  • Antoine Loquet
    • 5
  • Adam Lange
    • 5
  • Bernd Reif
    • 1
    • 2
    • 3
  1. 1.Helmholtz-Zentrum München (HMGU)Deutsches Forschungszentrum für Gesundheit und UmweltNeuherbergGermany
  2. 2.Munich Center for Integrated Protein Science (CIPS-M) at Department ChemieTechnische Universität München (TUM)GarchingGermany
  3. 3.Leibniz-Institut für Molekulare Pharmakologie (FMP)BerlinGermany
  4. 4.Bruker BioSpinRheinstettenGermany
  5. 5.Max-Planck-Institut für Biophysikalische ChemieGöttingenGermany
  6. 6.CIC EnergiguneMiñanoSpain