Journal of Biomolecular NMR

, Volume 56, Issue 4, pp 331–335

Divide and conquer is always best: sensitivity of methyl correlation experiments

  • Kaustubh Sinha
  • Linda Jen-Jacobson
  • Gordon S. Rule

DOI: 10.1007/s10858-013-9751-9

Cite this article as:
Sinha, K., Jen-Jacobson, L. & Rule, G.S. J Biomol NMR (2013) 56: 331. doi:10.1007/s10858-013-9751-9


The HMCM [CG]CBCA experiment (Tugarinov and Kay in J Am Chem Soc 125:13868–13878, 2003) correlates methyl carbon and proton shifts to Cγ, Cβ, and Cα resonances for the purpose of resonance assignments. The relative sensitivity of the HMCM[CG]CBCA sequence experiment is compared to a divide-and-conquer approach to assess whether it is best to collect all of the methyl correlations at once, or to perform separate experiments for each correlation. A straightforward analysis shows that the divide-and-conquer approach is intrinsically more sensitive, and should always be used to obtain methyl-Cγ, Cβ, and Cα correlations. The improvement in signal-to-noise associated with separate experiments is illustrated by the detection of methyl-aliphatic correlations in a 65 kDa protein-DNA complex.


Methyl resonance assignmentProtein-DNA complexPulse sequence

Supplementary material

10858_2013_9751_MOESM1_ESM.docx (240 kb)
Supplementary material 1 (DOCX 240 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Kaustubh Sinha
    • 1
  • Linda Jen-Jacobson
    • 2
  • Gordon S. Rule
    • 1
  1. 1.Department of Biological SciencesCarnegie Mellon UniversityPittsburghUSA
  2. 2.Department of Biological SciencesUniversity of PittsburghPittsburghUSA