Journal of Biomolecular NMR

, Volume 38, Issue 1, pp 11–22

Joint composite-rotation adiabatic-sweep isotope filtration

  • Elizabeth R. Valentine
  • Fabien Ferrage
  • Francesca Massi
  • David Cowburn
  • Arthur G. PalmerIII
Article

DOI: 10.1007/s10858-006-9131-9

Cite this article as:
Valentine, E.R., Ferrage, F., Massi, F. et al. J Biomol NMR (2007) 38: 11. doi:10.1007/s10858-006-9131-9

Abstract

Joint composite-rotation adiabatic-sweep isotope filters are derived by combining the composite-rotation [Stuart AC et al. (1999) J Am Chem Soc 121: 5346–5347] and adiabatic-sweep [Zwahlen C et al. (1997) J Am Chem Soc 119:6711–6721; Kupče E, Freeman R (1997) J Magn Reson 127:36–48] approaches. The joint isotope filters have improved broadband filtration performance, even for extreme values of the one-bond 1H–13C scalar coupling constants in proteins and RNA molecules. An average Hamiltonian analysis is used to describe evolution of the heteronuclear scalar coupling interaction during the adiabatic sweeps within the isotope filter sequences. The new isotope filter elements permit improved selective detection of NMR resonance signals originating from 1H spins attached to an unlabeled natural abundance component of a complex in which the other components are labeled with 13C and 15N isotopes.

Keywords

Composite pulseJ-filterNOESYStructure determinationvts SAM domain

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Elizabeth R. Valentine
    • 1
  • Fabien Ferrage
    • 2
    • 3
  • Francesca Massi
    • 1
  • David Cowburn
    • 2
  • Arthur G. PalmerIII
    • 1
  1. 1.Department of Biochemistry and Molecular BiophysicsColumbia UniversityNew YorkUSA
  2. 2.New York Structural Biology CenterNew YorkUSA
  3. 3.Département de ChimieCNRS UMR 8642 École normale supérieureParis Cedex 5France