Journal of Biomolecular NMR

, Volume 34, Issue 4, pp 245–257

Band-selective 13C Homonuclear 3D Spectroscopy for Solid Proteins at High Field with Rotor-synchronized Soft Pulses

  • Donghua H. Zhou
  • Kathryn D. Kloepper
  • Kem A. Winter
  • Chad M. Rienstra
Article

DOI: 10.1007/s10858-006-0026-6

Cite this article as:
Zhou, D.H., Kloepper, K.D., Winter, K.A. et al. J Biomol NMR (2006) 34: 245. doi:10.1007/s10858-006-0026-6

Abstract

We demonstrate improved 3D 13C–13C–13C chemical shift correlation experiments for solid proteins, utilizing band-selective coherence transfer, scalar decoupling and homonuclear zero-quantum polarization transfer. Judicious use of selective pulses and a z-filter period suppress artifacts with a two-step phase cycle, allowing higher digital resolution in a fixed measurement time. The novel correlation of Cali–Cali–CX (Cali for aliphatic carbons, CX for any carbon) reduces measurement time by an order of magnitude without sacrificing digital resolution. The experiment retains intensity from side-chain carbon resonances whose chemical shift dispersion is critical to minimize spectral degeneracy for large proteins with a predominance of secondary structure, such as β-sheet rich fibrillar proteins and α-helical membrane proteins. We demonstrate the experiment for the β1 immunoglobulin binding domain of protein G (GB1) and fibrils of the A30P mutant of α-synuclein, which is implicated in Parkinson’s disease. Selective pulses of duration comparable the rotor period give optimal performance, but must be synchronized with the spinning in non-trivial ways to minimize chemical shift anisotropy recoupling effects. Soft pulses with a small bandwidth-duration product are best for exciting the ~70 ppm bandwidth required for aliphatic-only dimensions.

Keywords

CCC 3DCSAdispersionresolutionselective pulsesolid-state NMR

Copyright information

© Springer 2006

Authors and Affiliations

  • Donghua H. Zhou
    • 1
  • Kathryn D. Kloepper
    • 1
  • Kem A. Winter
    • 1
  • Chad M. Rienstra
    • 1
  1. 1.Department of Chemistry, Department of Biochemistry, and Center for Biophysics and Computational BiologyUniversity of IllinoisUrbanaUSA