Skip to main content
SpringerLink
Log in

Detection of C′,Cα correlations in proteins using a new time- and sensitivity-optimal experiment

  • Article
  • Published:
Journal of Biomolecular NMR Aims and scope Submit manuscript

Abstract

Sensitivity- and time-optimal experiment, called COCAINE (CO-CA In- and aNtiphase spectra with sensitivity Enhancement), is proposed to correlate chemical shifts of 13C′ and 13Cα spins in proteins. A comparison of the sensitivity and duration of the experiment with the corresponding theoretical unitary bounds shows that the COCAINE experiment achieves maximum possible transfer efficiency in the shortest possible time, and in this sense the sequence is optimal. Compared to the standard HSQC, the COCAINE experiment delivers a 2.7-fold gain in sensitivity. This newly proposed experiment can be used for assignment of backbone resonances in large deuterated proteins effectively bridging 13C′ and 13Cα resonances in adjacent amino acids. Due to the spin-state selection employed, the COCAINE experiment can also be used for efficient measurements of one-bond couplings (e.g. scalar and residual dipolar couplings) in any two-spin system (e.g. the N/H in the backbone of protein).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • P. Andersson K. Nordstrand M. Sunnerhagen E. Liepinsh I. Turovskis G. Otting (1998) J. Biomol. NMR 11 445–450 Occurrence Handle10.1023/A:1008206212145 Occurrence Handle1:CAS:528:DyaK1cXkslSjsb8%3D Occurrence Handle9691285

    Article  CAS  PubMed  Google Scholar 

  • D. Braun K. Wüthrich G. Wider (2003) J. Magn. Reson. 165 89–94 Occurrence Handle1:CAS:528:DC%2BD3sXot1OitLc%3D Occurrence Handle14568519

    CAS  PubMed  Google Scholar 

  • F. Cordier A.J. Dingley S. Grzesiek (1999) J. Biomol. NMR 13 175–180 Occurrence Handle1:CAS:528:DyaK1MXhvVOgsbo%3D Occurrence Handle10070758

    CAS  PubMed  Google Scholar 

  • K.H. Gardner L.E. Kay (1998) Annu. Rev. Biophys. Biomolec. Struct. 27 357–406 Occurrence Handle1:CAS:528:DyaK1cXktVSltLk%3D

    CAS  Google Scholar 

  • S.J. Glaser T. Schulte-Herbruggen M. Sieveking O. Schedletzky N.C. Nielsen O.W. Sørensen C. Griesinger (1998) Science 280 421–424 Occurrence Handle1:CAS:528:DyaK1cXivVeltr8%3D Occurrence Handle9545217

    CAS  PubMed  Google Scholar 

  • S. Grzesiek J. Anglister H. Ren A. Bax (1993) J. Am. Chem. Soc. 115 4369–4370 Occurrence Handle1:CAS:528:DyaK3sXisVKju7k%3D

    CAS  Google Scholar 

  • S. Grzesiek H. Kuboniwa A.P. Hinck A. Bax (1995) J. Am. Chem. Soc. 117 5312–5315 Occurrence Handle1:CAS:528:DyaK2MXlsFCjs74%3D

    CAS  Google Scholar 

  • S. Grzesiek P. Wingfield S. Stahl J.D. Kaufman A. Bax (1995) J. Am. Chem. Soc. 117 9594–9595 Occurrence Handle1:CAS:528:DyaK2MXnvFOktrs%3D

    CAS  Google Scholar 

  • L.E. Kay P. Keifer T. Saarinen (1992) J. Am. Chem. Soc. 114 10663–10665 Occurrence Handle1:CAS:528:DyaK3sXjt1eg

    CAS  Google Scholar 

  • Khaneja, N., Brockett, R. and Glaser, S.J. (2001) Phys. Rev. A, 63, art. no. 032308

  • N. Khaneja B. Luy S.J. Glaser (2003a) Proc. Natl. Acad. Sci. USA 100 13162–13166 Occurrence Handle1:CAS:528:DC%2BD3sXptFOju74%3D

    CAS  Google Scholar 

  • N. Khaneja T. Reiss B. Luy S.J. Glaser (2003b) J. Magn. Reson. 162 311–319 Occurrence Handle1:CAS:528:DC%2BD3sXks1WgsL0%3D

    CAS  Google Scholar 

  • D.M. LeMaster (1994) Prog. Nucl. Magn. Reson. Spectrosc. 26 371–419 Occurrence Handle1:CAS:528:DyaK2cXktVCitL4%3D

    CAS  Google Scholar 

  • M.H. Lerche A. Meissner F.M. Poulsen O.W. Sørensen (1999) J. Magn. Reson. 140 259–263 Occurrence Handle1:CAS:528:DyaK1MXlslems70%3D Occurrence Handle10479570

    CAS  PubMed  Google Scholar 

  • F.A.A. Mulder A. Ayed D.W. Yang C.H. Arrowsmith L.E. Kay (2000) J. Biomol. NMR 18 173–176 Occurrence Handle1:CAS:528:DC%2BD3cXosVWgsLw%3D Occurrence Handle11101222

    CAS  PubMed  Google Scholar 

  • K. Pervushin A. Eletsky (2003) J. Biomol. NMR 25 147–152 Occurrence Handle1:CAS:528:DC%2BD3sXoslOntQ%3D%3D Occurrence Handle12652123

    CAS  PubMed  Google Scholar 

  • K. Pervushin R. Riek G. Wider K. Wüthrich (1997) Proc. Natl. Acad. Sci. USA 94 12366–12371 Occurrence Handle10.1073/pnas.94.23.12366 Occurrence Handle1:CAS:528:DyaK2sXns1ylsL8%3D Occurrence Handle9356455

    Article  CAS  PubMed  Google Scholar 

  • K. Pervushin R. Riek G. Wider K. Wüthrich (1998) J. Am. Chem. Soc. 120 6394–6400 Occurrence Handle1:CAS:528:DyaK1cXktFWjt7g%3D

    CAS  Google Scholar 

  • M. Piotto V. Saudek V. Sklenar (1992) J. Biomol. NMR 2 661–665 Occurrence Handle1:CAS:528:DyaK3sXitVyktrY%3D Occurrence Handle1490109

    CAS  PubMed  Google Scholar 

  • T.O. Reiss N. Khaneja S.J. Glaser (2002) J. Magn. Reson. 154 192–195 Occurrence Handle1:CAS:528:DC%2BD38XhtlelsLs%3D Occurrence Handle11846576

    CAS  PubMed  Google Scholar 

  • M. Salzmann K. Pervushin G. Wider H. Senn K. Wüthrich (1998) Proc. Natl. Acad. Sci. USA 95 13585–13590 Occurrence Handle1:CAS:528:DyaK1cXnsVGgsLc%3D Occurrence Handle9811843

    CAS  PubMed  Google Scholar 

  • M. Salzmann K. Pervushin G. Wider H. Senn K. Wüthrich (1999a) J. Biomol. NMR 14 85–88 Occurrence Handle1:CAS:528:DyaK1MXjvF2jtrs%3D

    CAS  Google Scholar 

  • M. Salzmann G. Wider K. Pervushin H. Senn K. Wüthrich (1999b) J. Am. Chem. Soc. 121 844–848 Occurrence Handle1:CAS:528:DyaK1MXnt1Ohsw%3D%3D

    CAS  Google Scholar 

  • T. Schulte-Herbruggen T.S. Untidt N.C. Nielsen O.W. Sørensen (2001) J. Chem. Phys. 115 8506–8517 Occurrence Handle1:CAS:528:DC%2BD3MXnvFWjs7s%3D

    CAS  Google Scholar 

  • Z. Serber C. Richter V. Dotsch (2001) Chembiochem 2 247–251 Occurrence Handle1:CAS:528:DC%2BD3MXivVCntbo%3D Occurrence Handle11828451

    CAS  PubMed  Google Scholar 

  • Z. Serber C. Richter D. Moskau J.M. Bohlen T. Gerfin D. Marek M. Haberli L. Baselgia F. Laukien A.S. Stern J.C. Hoch V. Dotsch (2000) J. Am. Chem. Soc. 122 3554–3555 Occurrence Handle1:CAS:528:DC%2BD3cXhvVykuro%3D

    CAS  Google Scholar 

  • X. Shan K.H. Gardner D.R. Muhandiram N.S. Rao C.H. Arrowsmith L.E. Kay (1996) J. Am. Chem. Soc. 118 6570–6579 Occurrence Handle1:CAS:528:DyaK28XjvVSisLY%3D

    CAS  Google Scholar 

  • T.E. Skinner T.O. Reiss B. Luy N. Khaneja S.J. Glaser (2003) J. Magn. Reson. 163 8–15 Occurrence Handle1:CAS:528:DC%2BD3sXlt1Wgt7k%3D Occurrence Handle12852902

    CAS  PubMed  Google Scholar 

  • M.D. Sørensen A. Meissner O.W. Sørensen (1999) J. Magn. Reson. 137 237–242 Occurrence Handle10053153

    PubMed  Google Scholar 

  • G.V.T. Swapna C.B. Rios Z.G. Shang G.T. Montelione (1997) J. Biomol. NMR 9 105–111 Occurrence Handle1:CAS:528:DyaK2sXhvVGltL8%3D

    CAS  Google Scholar 

  • T. Untidt T. Schulte-Herbruggen B. Luy S.J. Glaser C. Griesinger O.W. Sørensen N.C. Nielsen (1998) Mol. Phys. 95 787–796 Occurrence Handle1:CAS:528:DyaK1cXotVSqtL8%3D

    CAS  Google Scholar 

  • T.S. Untidt T. Schulte-Herbruggen O.W. Sørensen N.C. Nielsen (1999) J. Phys. Chem. A 103 8921–8926 Occurrence Handle1:CAS:528:DyaK1MXmslSnu74%3D

    CAS  Google Scholar 

  • Y.L. Xia X.M. Kong D.K. Smith Y. Liu D. Man G. Zhu (2000) J. Magn. Reson. 143 407–410 Occurrence Handle1:CAS:528:DC%2BD3cXhvVyjtr8%3D Occurrence Handle10729268

    CAS  PubMed  Google Scholar 

  • T. Yamazaki W. Lee C.H. Arrowsmith D.R. Muhandiram L.E. Kay (1994) J. Am. Chem. Soc. 116 11655–11666 Occurrence Handle1:CAS:528:DyaK2MXitlWntb0%3D

    CAS  Google Scholar 

  • D.W. Yang L.E. Kay (1999) J. Am. Chem. Soc. 121 2571–2575 Occurrence Handle1:CAS:528:DyaK1MXhsFarsbc%3D

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Physical Chemistry, ETH Hönggerberg, HCI, CH-8093, Zürich, Switzerland

    Donghan Lee, Beat Vögeli & Konstantin Pervushin

  2. NCI-Frederick, NIH Frederick, 21702, MD, USA

    Donghan Lee

Authors
  1. Donghan Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Beat Vögeli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Konstantin Pervushin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Donghan Lee or Konstantin Pervushin.

Electronic supplementary material

Electronic supplementary material

Supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, D., Vögeli, B. & Pervushin, K. Detection of C′,Cα correlations in proteins using a new time- and sensitivity-optimal experiment. J Biomol NMR 31, 273–278 (2005). https://doi.org/10.1007/s10858-005-2361-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10858-005-2361-4

Keywords

Search

Navigation