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In vitro biocompatibility assessment of Ti40Cu38Zr10Pd12 bulk metallic glass

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Abstract

The use of biocompatible materials has attained an increasing importance for tissue regeneration and transplantation. The excellent mechanical and corrosion properties of Ti40Cu38Zr10Pd12 bulk metallic glass (BMG) turn it into a potential candidate for its use in orthopaedic implants. Before being considered as a biomaterial, some biological parameters must be taken into account. In this study, mouse preosteoblasts were cultured in the presence or absence of the alloy at different times (24 h, 7 and 21 days) and no differences in cell viability were detected. Moreover, cells were able to adhere to the alloy surface by establishing focal contacts, and displayed a flattened polygonal morphology. After 14 days in culture, differentiation into osteoblasts was observed. Besides, the amount of Cu ions released and their potential toxic effects were analyzed, showing that the amount of Cu released did not increase cell death. Finally, the low levels of inflammatory cytokines secreted by THP-1 differentiated macrophages exposed to the alloy suggest the absence of an immunogenic response to the alloy. In conclusion, in vitro studies indicate that the Ti40Cu38Zr10Pd12 BMG could be considered as a biomaterial to be used in orthopaedic implants.

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Acknowledgments

This work has been partially financed by the Spanish Ministerio de Ciencia e Innovación (TEC2011-29140-C03-03), the Generalitat de Catalunya (2009-SGR-282 and 2009-SGR-1292) and the FP7-PEOPLE-2010-ITN-264635 (BioTiNet). A. Blanquer was supported by a predoctoral grant from the Universitat Autònoma de Barcelona. M. D. Baró was partially supported by an ICREA ACADEMIA award.

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Authors and Affiliations

  1. Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Edifici C, 08193, Bellaterra, Spain

    A. Blanquer, L. Barrios, E. Ibáñez & C. Nogués

  2. Departament de Física, Universitat Autònoma de Barcelona, Edifici C, 08193, Bellaterra, Spain

    E. Pellicer, A. Hynowska & M. D. Baró

  3. Institució Catalana de Recerca i Estudis Avançats (ICREA) and Departament de Física, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain

    J. Sort

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  1. A. Blanquer
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  2. E. Pellicer
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  3. A. Hynowska
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  4. L. Barrios
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Correspondence to E. Pellicer or C. Nogués.

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Blanquer, A., Pellicer, E., Hynowska, A. et al. In vitro biocompatibility assessment of Ti40Cu38Zr10Pd12 bulk metallic glass. J Mater Sci: Mater Med 25, 163–172 (2014). https://doi.org/10.1007/s10856-013-5041-z

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  • DOI: https://doi.org/10.1007/s10856-013-5041-z

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