Journal of Materials Science: Materials in Medicine

, Volume 24, Issue 2, pp 437–445

Zinc-substituted hydroxyapatite: a biomaterial with enhanced bioactivity and antibacterial properties

Authors

    • Department of Mechanical EngineeringNational University of Singapore
    • Kanagawa Academy of Science and Technology (KAST)
  • T. Konishi
    • Kanagawa Academy of Science and Technology (KAST)
  • Y. Kawanobe
    • Kanagawa Academy of Science and Technology (KAST)
    • Department of Applied Chemistry, School of Science and TechnologyMeiji University
  • P. N. Lim
    • Department of Mechanical EngineeringNational University of Singapore
  • C. Choong
    • School of Materials Science and EngineeringNanyang Technological University
  • B. Ho
    • Department of MircobiologyNational University of Singapore
  • M. Aizawa
    • Kanagawa Academy of Science and Technology (KAST)
    • Department of Applied Chemistry, School of Science and TechnologyMeiji University
Article

DOI: 10.1007/s10856-012-4817-x

Cite this article as:
Thian, E.S., Konishi, T., Kawanobe, Y. et al. J Mater Sci: Mater Med (2013) 24: 437. doi:10.1007/s10856-012-4817-x

Abstract

Hydroxyapatite (HA) is a synthetic biomaterial and has been found to promote new bone formation when implanted in a bone defect site. However, its use is often limited due to its slow osteointegration rate and low antibacterial activity, particularly where HA has to be used for long term biomedical applications. This work will describe the synthesis and detailed characterization of zinc-substituted HA (ZnHA) as an alternative biomaterial to HA. ZnHA containing 1.6 wt% Zn was synthesized via a co-precipitation reaction between calcium hydroxide, orthophosphoric acid and zinc nitrate hexahydrate. Single-phase ZnHA particles with a rod-like morphology measuring ~50 nm in length and ~15 nm in width, were obtained and characterized using transmission electron microscopy and X-ray diffraction. The substitution of Zn into HA resulted in a decrease in both the a- and c-axes of the unit cell parameters, thereby causing the HA crystal structure to alter. In vitro cell culture work showed that ZnHA possessed enhanced bioactivity since an increase in the growth of human adipose-derived mesenchymal stem cells along with the bone cell differentiation markers, were observed. In addition, antibacterial work demonstrated that ZnHA exhibited antimicrobial capability since there was a significant decrease in the number of viable Staphylococcus aureus bacteria after in contact with ZnHA.

Copyright information

© Springer Science+Business Media New York 2012