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Fluoride and calcium-phosphate coated sponges of the magnesium alloy AX30 as bone grafts: a comparative study in rabbits

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Abstract

Biocompatibility and degradation of magnesium sponges (alloy AX30) with a fluoride (MgF2 sponge, n = 24, porosity 63 ± 6 %, pore size 394 ± 26 μm) and with a fluoride and additional calcium-phosphate coating (CaP sponge, n = 24, porosity 6 ± 4 %, pore size 109 ± 37 μm) were evaluated over 6, 12 and 24 weeks in rabbit femurs. Empty drill holes (n = 12) served as controls. Clinical and radiological examinations, in vivo and ex vivo μ-computed tomographies and histological examinations were performed. Clinically both sponge types were tolerated well. Radiographs and XtremeCT evaluations showed bone changes comparable to controls and mild gas formation. The μCT80 depicted a higher and more inhomogeneous degradation of the CaP sponges. Histomorphometrically, the MgF2 sponges resulted in the highest bone and osteoid fractions and were integrated superiorly into the bone. Histologically, the CaP sponges showed more inflammation and lower vascularization. MgF2 sponges turned out to be better biocompatible and promising, biodegradable bone replacements.

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Acknowledgments

This study is part of the Collaborative Research Centre 599, which is funded by the German Research Foundation (DFG). Special thanks to Melanie Kielhorn, Diana Strauch and Svenja Pfarr for excellent technical support.

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Authors and Affiliations

  1. Small Animal Clinic, University of Veterinary Medicine Hannover, Bünteweg 9, 30559, Hannover, Germany

    Mareike Lalk, Janin Reifenrath & Nina Angrisani

  2. Department of Pathology, Dnipropetrovs’k State Medical Academy, Street Zhovtneva Ploshcha 14, Dnipropetrovs’k, 49005, Ukraine

    Alexandr Bondarenko

  3. Institute of Materials Science, Leibniz Universität Hannover, An der Universität 2, 30823, Garbsen, Germany

    Jan-Marten Seitz

  4. Department of Gene Regulation and Differentiation, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany

    Peter P. Mueller

  5. Clinic for Small Animal Surgery and Reproduction, Centre of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Ludwig Maximilian University Munich, Veterinärstraße 13, 80539, Munich, Germany

    Andrea Meyer-Lindenberg

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  1. Mareike Lalk
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  2. Janin Reifenrath
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Lalk, M., Reifenrath, J., Angrisani, N. et al. Fluoride and calcium-phosphate coated sponges of the magnesium alloy AX30 as bone grafts: a comparative study in rabbits. J Mater Sci: Mater Med 24, 417–436 (2013). https://doi.org/10.1007/s10856-012-4812-2

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