Journal of Materials Science: Materials in Medicine

, Volume 24, Issue 2, pp 479–488

A novel cyclic RGD-containing peptide polymer improves serum-free adhesion of adipose tissue-derived mesenchymal stem cells to bone implant surfaces

Authors

  • Péter Tátrai
    • Research Center for Natural Sciences, Hungarian Academy of Sciences
    • Department of Experimental Gene TherapyNational Blood Transfusion Service
  • Bernadett Sági
    • Stem Cell LaboratoryNational Blood Transfusion Service
  • Anna Szigeti
    • Creative Cell Ltd
  • Áron Szepesi
    • Creative Cell Ltd
  • Ildikó Szabó
    • Research Group of Peptide Chemistry, Hungarian Academy of Sciences
  • Szilvia Bősze
    • Research Group of Peptide Chemistry, Hungarian Academy of Sciences
  • Zoltán Kristóf
    • Department of Plant AnatomyEötvös Loránd University
  • Károly Markó
    • Institute of Experimental Medicine, Hungarian Academy of Sciences
  • Gergely Szakács
    • Research Center for Natural Sciences, Hungarian Academy of Sciences
  • István Urbán
    • Department of Restorative DentistryLoma Linda University
  • Gábor Mező
    • Research Group of Peptide Chemistry, Hungarian Academy of Sciences
  • Ferenc Uher
    • Stem Cell LaboratoryNational Blood Transfusion Service
    • Department of Experimental Gene TherapyNational Blood Transfusion Service
    • Creative Cell Ltd
Article

DOI: 10.1007/s10856-012-4809-x

Cite this article as:
Tátrai, P., Sági, B., Szigeti, A. et al. J Mater Sci: Mater Med (2013) 24: 479. doi:10.1007/s10856-012-4809-x

Abstract

Seeding of bone implants with mesenchymal stem cells (MSCs) may promote osseointegration and bone regeneration. However, implant material surfaces, such as titanium or bovine bone mineral, fail to support rapid and efficient attachment of MSCs, especially under serum-free conditions that may be desirable when human applications or tightly controlled experiments are envisioned. Here we demonstrate that a branched poly[Lys(Seri-DL-Alam)] polymer functionalized with cyclic arginyl-glycyl-aspartate, when immobilized by simple adsorption to tissue culture plastic, surgical titanium alloy (Ti6Al4V), or Bio-Oss® bovine bone substitute, significantly accelerates serum-free adhesion and enhances seeding efficiency of human adipose tissue-derived MSCs. Moreover, when exposed to serum-containing osteogenic medium, MSCs survived and differentiated on the peptide-coated scaffolds. In summary, the presented novel polypeptide conjugate can be conveniently used for coating various surfaces, and may find applications whenever quick and efficient seeding of MSCs is required to various scaffolds in the absence of serum.

Copyright information

© Springer Science+Business Media New York 2012