Journal of Materials Science: Materials in Medicine

, Volume 24, Issue 2, pp 381–393

Injectable collagen/α-tricalcium phosphate cement: collagen–mineral phase interactions and cell response


DOI: 10.1007/s10856-012-4799-8

Cite this article as:
Perez, R.A. & Ginebra, MP. J Mater Sci: Mater Med (2013) 24: 381. doi:10.1007/s10856-012-4799-8


A bone inspired material was obtained by incorporating collagen in the liquid phase of an α-tricalcium phosphate cement, either in solubilized or in fibrilized form. This material was able to set in situ, giving rise to a calcium deficient hydroxyapatite (CDHA)/collagen composite. The morphology and distribution of collagen in the composite was shown to be strongly affected by the collagen pre-treatment. The interactions between collagen and the inorganic phase were assessed by FTIR. A red shift of the amide I band was indicative of calcium chelation by the collagen carbonyl groups. The rate of CDHA formation was not affected when diluted collagen solutions (1 mg/ml) were used, whereas injectability improved. The presence of solubilized collagen, even in low amount (1 %), increased cell adhesion and proliferation on the composites. Still in the absence of osteogenic medium, significant ALP activity was detected both in the inorganic and the collagen-containing cements. The maximum ALP activity was advanced in the collagen-containing cement as compared to the inorganic cement.

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  1. 1.Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and MetallurgyTechnical University of Catalonia (UPC)BarcelonaSpain
  2. 2.Centre for Research in Nanoengineering, Technical University of Catalonia (UPC)BarcelonaSpain
  3. 3.Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)ZaragozaSpain