Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
The hypothesis for this study was that there is no difference in mesenchymal stem cells (MSCs) proliferation and osteogenic differentiation between calcium-phosphate (CaP) coatings with different crystal size deposited on different topographic surfaces of metal discs. Polished (P) and sand-blasted (SB) tantalum and TiAl6V4 discs were CaP coated by three methods—biomimetic (BioM), electrochemical at 20 mA/cm2 and at 6.5 mA/cm2—and cultured with MSCs. At days 4, 7 and 14, cell proliferation—alamarBlue® activity and DNA quantification—and differentiation down the osteogenic lineage—ALP activity normalised per amount of DNA and SEM (morphology)—were analysed. Results showed that MSCs proliferated more when cultured on the nano-sized BioM coatings compared to uncoated and electrochemically coated discs. MSCs also proliferated more on P surfaces than on SB and or electrochemical coatings. All the coatings induced osteogenic differentiation, which was greater on electrochemical coatings and SB discs.
- Matter P, Burch HB. Clinical experience with titanium implants, especially with the limited contact dynamic compression plate system. Arch Orthop Trauma Surg. 1990;109:311–3. CrossRef
- Grübl A, Chiari C, Gruber M, Kaider A, Gottsauner-Wolf F. Cementless total hip arthroplasty with a tapered, rectangular titanium stem and threaded cup: a minimum ten-year follow-up. J Bone Joint Surg Am. 2002;81-A(3):425–31.
- Unger AS, Lewis RJ, Gruen T. Evaluation of a porous tantalum uncemented acetabular cup in revision total hip arthroplasty: clinical and radiological results of 60 hips. J Arthroplasty. 2005;20(8):1002–9. CrossRef
- Levine BR, Sporer S, Poggie RA, Della Valle CJ, Jacobs JJ. Experimental and clinical performance of porous tantalum in orthopaedic surgery. Biomaterials. 2006;27:4671–81. CrossRef
- Blokhuis TJ, Termaat MF, den Boer FC, Patka P, Bakker FC, Haarman HJThM. Properties of calcium phosphate ceramics in relation to their in vivo behaviour. J Trauma Inj Infect Crit Care. 2000;48(1):179–86. CrossRef
- Tisdel CL, Goldberg VM, Parr JA, Bensusan JS, Staikoff LS, Stevenson S. The influence of a hydroxyapatite and tricalcium-phosphate coating on bone growth into titanium fiber-metal implants. J Bone Joint Surg Am. 1994;76-A(2):159–71.
- Kokubo T, Kushitani H, Sakka S, Kitsugi T, Yamamuro T. Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W. J Biomed Mater Res. 1990;24:721–34. CrossRef
- Kokubo T. Apatite formation on surfaces of ceramics, metals and polymers in body environment. Acta Mater. 1998;46(7):2519–27. CrossRef
- Habibovic P, Barrère F, van Blitterswijk CA, de Groot K, Layrolle P. Biomimetic hydroxyapatite coating on metal implants. J Am Ceram Soc. 2002;85(3):517–22. CrossRef
- Tas AC, Bhaduri SB. Rapid coating of Ti6Al4V at room temperature with a calcium phosphate solution similar to 10× simulated body fluid. J Mater Res. 2004;19(9):2742–9. CrossRef
- Bharati S, Sinha MK, Basu D. Hydroxyapatite coating by biomimetic method on titanium alloy using concentrated SBF. Bull Mater Sci. 2005;28(6):617–21. CrossRef
- Redepenning J, Schlessinger T, Burnham S, Lippiello L, Miyano J. Characterization of electrolytically prepared brushite and hydroxyapatite coatings on orthopaedic alloys. J Biomed Mater Res. 1996;30:287–94. CrossRef
- Kashima DP, Rakngarm A. Calcium phosphate film coating on titanium substrate by electrochemical deposition. J Met Mater Miner. 2008;18(1):27–31.
- Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284:143–7. CrossRef
- Ohgushi H, Dohi Y, Tamai S, Tabata S. Osteogenic differentiation of marrow stromal stem cells in porous hydroxyapatite ceramics. J Biomed Mater Res. 1993;27:1401–7. CrossRef
- Oreffo ROC, Driessens FCM, Planell JA, Triffitt JT. Growth and differentiation of human bone marrow osteoprogenitors on novel calcium phosphate cements. Biomaterials. 1998;19:1845–54. CrossRef
- Nishio K, Neo M, Akiyama H, Nishiguchi S, Kim HM, Kokubo T, Nakamura T. The effect of alkali-and heat-treated titanium and apatite-formed titanium on osteoblastic differentiation of bone marrow cells. J Biomed Mater Res. 2000;52(4):652–61. CrossRef
- Anselme K, Bigerelle M, Noel B, Dufresne E, Judas D, Iost A, Hardouin P. Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses. J Biomed Mater Res. 2000;49(2):155–66. CrossRef
- Weiβenböck M, Stein E, Undt G, Ewers R, Lauer G, Turhani D. Particle size of hydroxyapatite granules calcified from red algae affects the osteogenic potential of human mesenchymal stem cells in vitro. Cells Tissues Organs. 2006;182:79–88. CrossRef
- Chen F, Lam WM, Lin CJ, Qiu GX, Wu ZH, Luk KDK, Lu WW. Biocompatibility of electrophoretical deposition of nanostructured hydroxyapatite coating on roughen titanium surface: in vitro evaluation using mesenchymal stem cells. J Biomed Mater Res. 2007;82B:183–91. CrossRef
- Kokubo T, Kim HM, Kawashita M, Nakamura T. Process of calcification on artificial materials. Z Kardiol. 2001;90(3):86–91.
- Li J, Liao H, Sjöström M. Characterization of calcium phosphates precipitated from simulated body fluid of different buffering capacities. Biomaterials. 1997;18:743–7. CrossRef
- Zhang Q, Chen J, Feng J, Cao Y, Deng C, Zhang X. Dissolution and mineralisation behaviours of HA coatings. Biomaterials. 2003;24:4741–8. CrossRef
- Wopenka B, Pasteris JD. A mineralogical perspective on the apatite in bone. Mater Sci Eng. 2005;C25:131–43.
- LeGeros RZ. Biodegradation and bioresorption of calcium phosphate ceramics. Clin Mater. 1993;14:65–88. CrossRef
- Hara M, Murakami T, Kobayashi E. In vivo bioimaging using photogenic rats: fate of injected bone marrow-derived mesenchymal stromal cells. J Autoimmunity. 2008;30:163–71. CrossRef
- Erices A, Conget P, Minguell JJ. Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol. 2000;109:235–42. CrossRef
- Vrouwenvelder WCA, Groot CG, de Groot K. Histological and biochemical evaluation of osteoblasts cultured on bioactive glass, hydroxylapatite, titanium alloy and stainless steel. J Biomed Mater Res. 1993;27:465–75. CrossRef
- Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP. Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem. 1997;64:295–312. CrossRef
- Bruder SP, Jaiswal N, Haynesworth SE. Growth kinetics, self-renewal and the osteogenic potential of purified human mesenchynal stem cells during extensive subcultivation and following cryopreservation. J Cell Biochem. 1997;64:278–94. CrossRef
- Lian JB, Stein GS. Concepts of osteoblast growth and differentiation: basis for modulation of bone cell development and tissue formation. Crit Rev Oral Biol Med. 1992;3(3):269–305.
- Ergun C, Liu H, Halloran JW, Webster TJ. Increased osteoblasts adhesion on nanograined hydroxyapatite and tricalcium phosphate containing calcium titanate. J Biomed Mater Res. 2007;80A:990–7. CrossRef
- Ohgushi H, Dohi Y, Yoshikawa T, Tamai S, Tabata S, Okunaga K, Shibuya T. Osteogenic differentiation of cultured marrow stromal stem cells on the surface of bioactive glass ceramics. J Biomed Mater Res. 1996;32:341–8. CrossRef
- Jäger M, Urselmann F, Witte F, Zanger K, Li X, Ayers DC, Krauspe R. Osteoblast differentiation onto different biometals with an endoprosthetic surface topography in vitro. J Biomed Mater Res. 2008;86A:61–75. CrossRef
- Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces
Journal of Materials Science: Materials in Medicine
Volume 24, Issue 1 , pp 199-210
- Cover Date
- Print ISSN
- Online ISSN
- Springer US
- Additional Links
- Industry Sectors
- Author Affiliations
- 1. John Scales Centre for Biomedical Engineering, Institute of Orthopaedics and Musculoskeletal Science, University College London, Royal National Orthopaedic Hospital, Stanmore, HA7 4LP, UK
- 2. Eastman Dental Institute, University College London, 256 Gray’s Inn Road, London, WC1X 8LD, UK