Article

Journal of Materials Science: Materials in Medicine

, Volume 23, Issue 8, pp 1835-1847

Structural and biomechanical characterizations of porcine myocardial extracellular matrix

  • Bo WangAffiliated withTissue Bioengineering Laboratory, Department of Agricultural and Biological Engineering, Computational Manufacturing and Design, CAVS, Mississippi State University
  • , Mary E. TedderAffiliated withDepartment of Bioengineering, Clemson University
  • , Clara E. PerezAffiliated withTissue Bioengineering Laboratory, Department of Agricultural and Biological Engineering, Computational Manufacturing and Design, CAVS, Mississippi State University
  • , Guangjun WangAffiliated withTissue Bioengineering Laboratory, Department of Agricultural and Biological Engineering, Computational Manufacturing and Design, CAVS, Mississippi State University
  • , Amy L. de Jongh CurryAffiliated withDepartment of Biomedical Engineering, University of Memphis
  • , Filip ToAffiliated withTissue Bioengineering Laboratory, Department of Agricultural and Biological Engineering, Computational Manufacturing and Design, CAVS, Mississippi State University
  • , Steven H. ElderAffiliated withTissue Bioengineering Laboratory, Department of Agricultural and Biological Engineering, Computational Manufacturing and Design, CAVS, Mississippi State University
  • , Lakiesha N. WilliamsAffiliated withTissue Bioengineering Laboratory, Department of Agricultural and Biological Engineering, Computational Manufacturing and Design, CAVS, Mississippi State University
  • , Dan T. SimionescuAffiliated withDepartment of Bioengineering, Clemson University
    • , Jun LiaoAffiliated withTissue Bioengineering Laboratory, Department of Agricultural and Biological Engineering, Computational Manufacturing and Design, CAVS, Mississippi State University Email author 

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Abstract

Extracellular matrix (ECM) of myocardium plays an important role to maintain a multilayered helical architecture of cardiomyocytes. In this study, we have characterized the structural and biomechanical properties of porcine myocardial ECM. Fresh myocardium were decellularized in a rotating bioreactor using 0.1 % sodium dodecyl sulfate solution. Masson’s trichrome staining and SEM demonstrated the removal of cells and preservation of the interconnected 3D cardiomyocyte lacunae. Movat’s pentachrome staining showed the preservation of cardiac elastin ultrastructure and vascular elastin distribution/alignment. DNA assay result confirmed a 98.59 % reduction in DNA content; the acellular myocardial scaffolds were found completely lack of staining for the porcine α-Gal antigen; and the accelerating enzymatic degradation assessment showed a constant degradation rate. Tensile and shear properties of the acellular myocardial scaffolds were also evaluated. Our observations showed that the acellular myocardial ECM possessed important traits of biodegradable scaffolds, indicating the potentials in cardiac regeneration and whole heart tissue engineering.