Journal of Materials Science: Materials in Medicine

, Volume 23, Issue 8, pp 1835–1847

Structural and biomechanical characterizations of porcine myocardial extracellular matrix

Authors

  • Bo Wang
    • Tissue Bioengineering Laboratory, Department of Agricultural and Biological Engineering, Computational Manufacturing and DesignCAVS, Mississippi State University
  • Mary E. Tedder
    • Department of BioengineeringClemson University
  • Clara E. Perez
    • Tissue Bioengineering Laboratory, Department of Agricultural and Biological Engineering, Computational Manufacturing and DesignCAVS, Mississippi State University
  • Guangjun Wang
    • Tissue Bioengineering Laboratory, Department of Agricultural and Biological Engineering, Computational Manufacturing and DesignCAVS, Mississippi State University
  • Amy L. de Jongh Curry
    • Department of Biomedical EngineeringUniversity of Memphis
  • Filip To
    • Tissue Bioengineering Laboratory, Department of Agricultural and Biological Engineering, Computational Manufacturing and DesignCAVS, Mississippi State University
  • Steven H. Elder
    • Tissue Bioengineering Laboratory, Department of Agricultural and Biological Engineering, Computational Manufacturing and DesignCAVS, Mississippi State University
  • Lakiesha N. Williams
    • Tissue Bioengineering Laboratory, Department of Agricultural and Biological Engineering, Computational Manufacturing and DesignCAVS, Mississippi State University
  • Dan T. Simionescu
    • Department of BioengineeringClemson University
    • Tissue Bioengineering Laboratory, Department of Agricultural and Biological Engineering, Computational Manufacturing and DesignCAVS, Mississippi State University
Article

DOI: 10.1007/s10856-012-4660-0

Cite this article as:
Wang, B., Tedder, M.E., Perez, C.E. et al. J Mater Sci: Mater Med (2012) 23: 1835. doi:10.1007/s10856-012-4660-0

Abstract

Extracellular matrix (ECM) of myocardium plays an important role to maintain a multilayered helical architecture of cardiomyocytes. In this study, we have characterized the structural and biomechanical properties of porcine myocardial ECM. Fresh myocardium were decellularized in a rotating bioreactor using 0.1 % sodium dodecyl sulfate solution. Masson’s trichrome staining and SEM demonstrated the removal of cells and preservation of the interconnected 3D cardiomyocyte lacunae. Movat’s pentachrome staining showed the preservation of cardiac elastin ultrastructure and vascular elastin distribution/alignment. DNA assay result confirmed a 98.59 % reduction in DNA content; the acellular myocardial scaffolds were found completely lack of staining for the porcine α-Gal antigen; and the accelerating enzymatic degradation assessment showed a constant degradation rate. Tensile and shear properties of the acellular myocardial scaffolds were also evaluated. Our observations showed that the acellular myocardial ECM possessed important traits of biodegradable scaffolds, indicating the potentials in cardiac regeneration and whole heart tissue engineering.

Copyright information

© Springer Science+Business Media, LLC 2012