A novel in vitro model for preclinical testing of the hemocompatibility of intravascular stents according to ISO 10993-4

  • Stefan Sinn
  • Torsten Scheuermann
  • Stephan Deichelbohrer
  • Gerhard Ziemer
  • Hans P. Wendel
Article

DOI: 10.1007/s10856-011-4335-2

Cite this article as:
Sinn, S., Scheuermann, T., Deichelbohrer, S. et al. J Mater Sci: Mater Med (2011) 22: 1521. doi:10.1007/s10856-011-4335-2

Abstract

Subacute stent thrombosis, caused by undesired interactions between blood and the stent surface, is a major concern in the first few weeks following coronary artery stent implantation. The aim of this study was to establish a novel in vitro model for hemocompatibility testing of coronary artery stents according to ISO 10993-4. The model consists of a modified Chandler-Loop design with closed heparin-coated PVC Loops and a thermostated water bath. The tests were performed with anticoagulated human whole blood. After incubation in the loop, blood was analyzed for coagulation and inflammatory activation markers (TAT, β-TG, sP-selectin, SC5b-9 and PMN-elastase). Three different stent types with varying thrombogenicity were tested; statistically significant differences were found between the three stent types in measures of coagulation and platelet activation. The new Chandler-Loop model can be used as an alternative to animal and current in vitro models, especially for the determination of early events after stent implantation.

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Stefan Sinn
    • 1
  • Torsten Scheuermann
    • 2
  • Stephan Deichelbohrer
    • 1
  • Gerhard Ziemer
    • 1
  • Hans P. Wendel
    • 1
  1. 1.Division of Congenital & Pediatric Cardiac SurgeryUniversity Children’s Hospital, Clinical Research LaboratoryTubingenGermany
  2. 2.Boston Scientific Technologie Zentrum GmbHMunichGermany