In vitro photothermal study of gold nanoshells functionalized with small targeting peptides to liver cancer cells
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Gold nanoshells functionalized with a small peptide as a targeting agent were designed and synthesized for photothermal therapy of hepatocarcinoma. The nanoshells exhibited high absorption in the near-infrared (NIR) range, 800–1,100 nm, and were functionalized with 12-amino acid sequence peptides for targeting liver cancer cells. The nanoshells were characterized by Dynamic Light Scattering (DLS), Transmission Electron Microscope (TEM) and IR spectra. The functionalized gold nanoshells showed good targeting ability to liver cancer cells BEL-7404 and BEL-7402 while not to the normal healthy liver cell HL-7702, and also had a low cytotoxic activity. The fluorescence images showed that the gold nanoshells caused death to the liver cancer cells efficiently after being treated with a NIR light in vitro. These simple, stable, low cytotoxic, cancer-cell targeting gold nanoshells present a great promise as delivery agents for the selective photothermal treatment of liver cancer cells.
- Pisani P, Parkin DM, Bray F, Ferlay J. Estimates of the worldwide mortality from 25 cancers in 1990. Int J Cancer. 1999;83:18–29. CrossRef
- Sachdeva MS. Drug targeting systems for cancer chemotherapy. Expert Opin Invest Drugs. 1998;7:1849–64. CrossRef
- Torchilin VP. Drug targeting. Eur J Pharm Sci. 2000;11:81–91. CrossRef
- Hirsch LR, Stafford RJ, Bankson JA, Sershen SR, Halas NJ. Nanoshell-mediated near-infrared thermal therapy of tumors under magnetic resonance guidance. Proc Natl Acad Sci. 2003;100:13549–54. CrossRef
- Gobin AM, Lee MH, Halas NJ, James WD. Near-infrared resonant nanoshells for combined optical imaging and photothermal cancer therapy. Nano Lett. 2007;7:1929–34. CrossRef
- Maltzahn G, Park J-H, Agrawal A, Bandaru NK, Das SK, Sailor MJ, et al. Computationally cuided photothermal tumor therapy using long-circulating gold nanorod antennas. Cancer Res. 2009;69:3892–9. CrossRef
- Lal S, Clare SE, Halas NJ. Nanoshell-enabled photothermal cancer therapy: impending clinical inpact. Acc Chem Res. 2008;41:1842–51. CrossRef
- Loo C, Hirsch L, Lee MH, Chang E, West J, Halas NJ, et al. Gold nanoshell bioconjugates for molecular imaging in living cells. Opt Lett. 2005;30:1012–4. CrossRef
- Oldenburg SJ, Jackson JB, Westcott SL, Halas NJ. Infrared extinction properties of gold nanoshells. Appl Phys Lett. 1999;111:2897–9. CrossRef
- Hirsch LR, Gobin AM, Lowery AR, Drezek RA, Halas NJ, West JL. Metal nanoshells. Ann Biomed Eng. 2006;34:15–22. CrossRef
- Gobin AM, O’Neal DP, Watkins DM, Halas NJ, Drezek RA, West JL. Near infrared laser-tissue welding using nanoshells as an exogenous absorber. Lasers Surg Med. 2005;9999:1–7.
- O’Neal DP, Hirsch LR, Halas NJ, Payne JD, West JL. Photo-thermal tumor ablation in mice using near infrared-absorbing nanoparticles. Cancer Lett. 2004;209:171–6. CrossRef
- Hashizume H, Baluk P, Morikawa S, McLean JW. Openings between defective endothelial cells explain tumor vessel leakiness. Am J Pathol. 2000;156:1363–80.
- Loo C, Lowery A, Halas NJ, West JL, Drezek R. Immunotargeted nanoshells for integrated cancer imaging and therapy. Nano Lett. 2005;5:709–11. CrossRef
- Lowery AR, Gobin AM, Day ES, Halas NJ, West JL. Immunonanoshells for targeted photothermal ablation of tumor cells. Int J Nanomed. 2006;1:149–54. CrossRef
- Lin AWH, Lewinski NA, West JL. Optically tunable nanoparticle contrast agents for early cancer detection: model-based analysis of gold nanoshells. J Biomed Opt. 2005;10:064035-1-10.
- Diagaradjane P, Shetty A, Wang JC, Elliott AM, Schwartz J, Shentu S, et al. Modulation of in vivo tumor radiation response via gold nanoshell-mediated vascular-focused hyperthermia: characterizing an integrated antihypoxic and localized vascular disrupting targeting strategy. Nano Lett. 2008;8:1492–500. CrossRef
- Dixit V, Bossche JV, Sherman DM, Thompson DH. Synthesis and grafting of thioctic acid-PEG-folate conjugates onto Au nanoparticles for selective targeting of folate receptor-positive tumor cells. Bioconjugate Chem. 2006;17:603–9. CrossRef
- Wang SN, Deng YH, Xu H, Wu HB, Qiu YK, Chen DW. Synthesis of a novel galactosylated lipid and its application to the hepatocyte-selective targeting of liposomal doxorubicin. Eur J Pharma Biopharma. 2006;62:32–8. CrossRef
- Otte A, Mueller-Brand J, Dellas S, Nitzsche EU, Herrmann R, Maecke HR. Yttrium-90-labelled somatostatin-analogue for cancer treatment. Lancet. 1998;351:417–8. CrossRef
- Yang Y, Jiang J-S, Du B, Gan Z-F, Qian M, Zhang P. Preparation and properties of a novel drug delivery system with both magnetic and biomolecular targeting. J Mater Sci Mater Med. 2009;20:301–7. CrossRef
- Pham T, Jackson JB, Halas NJ, Lee TR. Preparation and characterization of gold nanoshells coated with self-assembled monolayers. Langmuir. 2002;18:4915–20. CrossRef
- Stöber W, Fink A. Controlled growth of monodisperse silica spheres in the micron size range. J Colloid Interface Sci. 1968;26:62–9. CrossRef
- Duff DG, Baiker A. A new hydrosol of gold clusters. 1. Formation and particle size variation. Langmuir. 1993;9:2301–9. CrossRef
- Kong G, Braun RD, Dewhirst MW. Hyperthermia enables tumor-specific nanoparticle delivery: effect of particle size. Cancer Res. 2000;60:4440–5.
- Ishida O, Maruyama K, Sasaki K, Iwatsuru M. Size-dependent extravasation and interstitial localization of polyethyleneglycol liposomes in solid tumor-bearing mice. Int J Pharm. 1999;190:49–56. CrossRef
- Hobbs SK, Monsky WL, Yuan F, Roberts WG, Griffith L, Torchilin VP, et al. Regulation of transport pathways in tumor vessels: role of tumor type and microenvironment. Proc Natl Acad Sci. 1998;95:4607–12. CrossRef
- Birnboim MH, Ma WP. Nonlinear optical properties of structured nanoparticle composites. Mater Res Soc Symp Proc. 1990;164:277–82.
- Wang YJ, Zhu SG, Xun CF. Biochemistry. 3rd ed. Beijing, China: Higher Education Press; 2003.
- Fuente JM, Berry CC, Riehle MO. Nanoparticle targeting at cells. Langmuir. 2006;22:3286–93. CrossRef
- Chen JY, Saeki F, Wiley BJ. Gold nanocages: bioconjugation and their potential use as optical imaging contrast agents. Nano Lett. 2005;5:473–7. CrossRef
- Young JK, Chong RP. Analysis of problematic complexing behavior of ferric chloride with N,N-dimethylformamide using combined techniques of FT-IR, XPS, and TGA/DTG. Inorg Chem. 2002;41:6211–6. CrossRef
- Carmen T, Bogdan G, Vasile IP, Víctor LF, André G, Michael UK. Investigation of the hydrophobization efficiency of terbium-exchanged BEA zeolites by means of FT-IR, TGA, physical adsorption, and time-resolved photoluminescence. Langmuir. 2007;23:6781–7. CrossRef
- Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55–63. CrossRef
- Sgouras D, Duncan R. Methods for the evaluation of biocompatibility of soluble synthetic polymers which have potential for biomedical use: 1-Use of the tetrazolium-based colorimetric assay (MTT) as a preliminary screen for evaluation ofin vitro cytotoxicity. J Mater Sci: Mater Med. 1990;1:67–8. CrossRef
- Homa J, Bzowska M, Klimek M, Plytycz B. Flow cytometric quantification of proliferating coelomocytes non-invasively retrieved from the earthworm, Dendrobaena veneta. Dev Comp Immunol. 2008;32:9–14. CrossRef
- Jin C, Bai L, Wu H, Tian F, Guo G. Radiosensitization of paclitaxel, etanidazole and paclitaxel+etanidazole nanoparticles on hypoxic human tumor cells in vitro. Biomaterials. 2007;28:3724–30. CrossRef
- Kah JCY, Wan RCY, Wong KY, Mhaisalkar S, Sheppard CJR, Olivo M. Combinatorial treatment of photothermal therapy using cold nanoshells with conventional photodynamic therapy to improve treatment efficacy: an in vitro study. Lasers Surg Med. 2008;40:584–9. CrossRef
- Haveman J, Hahn GM. The role of energy in dyperthermia-induced mammalian cell inactivation: a study of the effects of glucose starvation and an uncoupler of oxidative phosphorylation. J Cell Physiol. 1981;107:237–41. CrossRef
- Ishiguro K, Hatcho M, Miyoshi N, Fukuda M, Ueda K. Microfluorocytometric detection of nuclear DNA damage to cancer cells in squamous cell carcinoma after hyperthermia. J Dermatol. 1994;21:92–7.
- In vitro photothermal study of gold nanoshells functionalized with small targeting peptides to liver cancer cells
Journal of Materials Science: Materials in Medicine
Volume 21, Issue 2 , pp 665-674
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- 1. Advanced Research Center of NBIC Integrated Drug Discovery and Development, East China Normal University, Shanghai, 200062, China
- 2. Department of Materials Science and Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA