Journal of Materials Science: Materials in Medicine

, 20:1571

Macroporous hydrogels based on 2-hydroxyethyl methacrylate. Part 6: 3D hydrogels with positive and negative surface charges and polyelectrolyte complexes in spinal cord injury repair

Authors

    • Institute of Experimental MedicineAcademy of Sciences of the Czech Republic
    • Department of Neuroscience and Centre for Cell Therapy and Tissue Repair, 2nd Medical Faculty, Faculty Hospital MotolCharles University
    • Department of NeurosurgeryMasaryk Hospital
  • P. Lesný
    • Institute of Experimental MedicineAcademy of Sciences of the Czech Republic
    • Department of Neuroscience and Centre for Cell Therapy and Tissue Repair, 2nd Medical Faculty, Faculty Hospital MotolCharles University
  • M. Přádný
    • Department of Neuroscience and Centre for Cell Therapy and Tissue Repair, 2nd Medical Faculty, Faculty Hospital MotolCharles University
    • Institute of Macromolecular ChemistryAcademy of Sciences of the Czech Republic
  • J. Šedý
    • Institute of Experimental MedicineAcademy of Sciences of the Czech Republic
    • Department of Neuroscience and Centre for Cell Therapy and Tissue Repair, 2nd Medical Faculty, Faculty Hospital MotolCharles University
  • J. Zámečník
    • Department of Pathology and Molecular Medicine, 2nd Medical Faculty, Faculty Hospital MotolCharles University
  • P. Jendelová
    • Institute of Experimental MedicineAcademy of Sciences of the Czech Republic
    • Department of Neuroscience and Centre for Cell Therapy and Tissue Repair, 2nd Medical Faculty, Faculty Hospital MotolCharles University
  • J. Michálek
    • Department of Neuroscience and Centre for Cell Therapy and Tissue Repair, 2nd Medical Faculty, Faculty Hospital MotolCharles University
    • Institute of Macromolecular ChemistryAcademy of Sciences of the Czech Republic
  • E. Syková
    • Institute of Experimental MedicineAcademy of Sciences of the Czech Republic
    • Department of Neuroscience and Centre for Cell Therapy and Tissue Repair, 2nd Medical Faculty, Faculty Hospital MotolCharles University
Article

DOI: 10.1007/s10856-009-3714-4

Cite this article as:
Hejčl, A., Lesný, P., Přádný, M. et al. J Mater Sci: Mater Med (2009) 20: 1571. doi:10.1007/s10856-009-3714-4

Abstract

Macroporous hydrogels are artificial biomaterials commonly used in tissue engineering, including central nervous system (CNS) repair. Their physical properties may be modified to improve their adhesion properties and promote tissue regeneration. We implanted four types of hydrogels based on 2-hydroxyethyl methacrylate (HEMA) with different surface charges inside a spinal cord hemisection cavity at the Th8 level in rats. The spinal cords were processed 1 and 6 months after implantation and histologically evaluated. Connective tissue deposition was most abundant in the hydrogels with positively-charged functional groups. Axonal regeneration was promoted in hydrogels carrying charged functional groups; hydrogels with positively charged functional groups showed increased axonal ingrowth into the central parts of the implant. Few astrocytes grew into the hydrogels. Our study shows that HEMA-based hydrogels carrying charged functional groups improve axonal ingrowth inside the implants compared to implants without any charge. Further, positively charged functional groups promote connective tissue infiltration and extended axonal regeneration inside a hydrogel bridge.

Copyright information

© Springer Science+Business Media, LLC 2009