Article

Journal of Materials Science: Materials in Medicine

, Volume 19, Issue 12, pp 3525-3533

First online:

PEG-grafted chitosan nanoparticles as an injectable carrier for sustained protein release

  • X. G. ZhangAffiliated withKey Laboratory of Functional Polymer Materials, Ministry Education, and Institute of Polymer Chemistry, Nankai University
  • , D. Y. TengAffiliated withKey Laboratory of Functional Polymer Materials, Ministry Education, and Institute of Polymer Chemistry, Nankai University
  • , Z. M. WuAffiliated withMetabolic Diseases Hospital, Tianjin Medical University Email author 
  • , X. WangAffiliated withKey Laboratory of Functional Polymer Materials, Ministry Education, and Institute of Polymer Chemistry, Nankai University
  • , Z. WangAffiliated withKey Laboratory of Functional Polymer Materials, Ministry Education, and Institute of Polymer Chemistry, Nankai University
  • , D. M. YuAffiliated withMetabolic Diseases Hospital, Tianjin Medical University
  • , C. X. LiAffiliated withKey Laboratory of Functional Polymer Materials, Ministry Education, and Institute of Polymer Chemistry, Nankai University Email author 

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Abstract

The development of injectable nanoparticulate “stealth” carriers for protein delivery is a major challenge. The objective of this work was to investigate the possibility of achieving the controlled release of a model protein, insulin, from PEG-grafted chitosan (PEG-g-chitosan) nanoparticles (mean diameter 150–300 nm) prepared by the ion gelation method. Insulin was efficiently incorporated into the nanoparticles, and reached as high as 38%. In vitro release showed that it could control the insulin release by choosing the composition, loading and release temperature. We observed that the composition of the nanoparticle surface (C/O ratio) increased from 2.40 to 3.23, with an increase in the incubation time. Therefore, we concluded that during this time, insulin release from PEG-g-chitosan nanoparticles followed a diffusion mechanism in which erosion was negligible. The experiments also demonstrated that PEG-g-chitosan helped to maintain the natural structure of the protein entrapped in the nanoparticles.