Journal of Materials Science: Materials in Medicine

, Volume 19, Issue 11, pp 3399–3409

Silkworm and spider silk scaffolds for chondrocyte support

  • Kris Gellynck
  • Peter C. M. Verdonk
  • Els Van Nimmen
  • Karl F. Almqvist
  • Tom Gheysens
  • Gustaaf Schoukens
  • Lieva Van Langenhove
  • Paul Kiekens
  • Johan Mertens
  • Gust Verbruggen
Article

DOI: 10.1007/s10856-008-3474-6

Cite this article as:
Gellynck, K., Verdonk, P.C.M., Van Nimmen, E. et al. J Mater Sci: Mater Med (2008) 19: 3399. doi:10.1007/s10856-008-3474-6

Abstract

Objective To create scaffolds with silkworm cocoon, spider egg sac and spider dragline silk fibres and examine their use for chondrocyte attachment and support. Methods Three different kinds of scaffolds were developed with Bombyx mori cocoon, Araneus diadematus egg sac and dragline silk fibres. The attachment of human articular cartilage cells were investigated on these bioprotein matrices. The chondrocytes produced an extracellular matrix which was studied by immunostaining. Moreover, the compression behaviour in relation to the porosity was studied. Results The compression modulus of a silkworm silk scaffold was related to its porosity. Chondrocytes were able to attach and to grow on the different fibres and in the scaffolds for several weeks while producing extracellular matrix products. Conclusion Porous scaffolds can be made out of silkworm and spider silk for cartilage regeneration. Mechanical properties are related to porosity and pore size of the construct. Cell spreading and cell expression depended on the porosity and pore-size.

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Kris Gellynck
    • 1
  • Peter C. M. Verdonk
    • 2
  • Els Van Nimmen
    • 1
  • Karl F. Almqvist
    • 2
  • Tom Gheysens
    • 3
  • Gustaaf Schoukens
    • 1
  • Lieva Van Langenhove
    • 1
  • Paul Kiekens
    • 1
  • Johan Mertens
    • 3
  • Gust Verbruggen
    • 4
  1. 1.Faculty of Engineering, Department of TextilesGhent UniversityZwijnaardeBelgium
  2. 2.Department of Orthopaedic SurgeryGhent University HospitalGhentBelgium
  3. 3.Faculty of Sciences, Department of Biology, Terrestrial EcologyGhent UniversityGhentBelgium
  4. 4.Department of RheumatologyGhent University HospitalGhentBelgium