Journal of Materials Science: Materials in Medicine

, Volume 19, Issue 2, pp 827-832

First online:

Encapsulation and controlled release of lysozyme from electrospun poly(ε-caprolactone)/poly(ethylene glycol) non-woven membranes by formation of lysozyme–oleate complexes

  • Yan LiAffiliated withDepartment of Polymer Science and Engineering, Zhejiang University
  • , Hongliang JiangAffiliated withDepartment of Polymer Science and Engineering, Zhejiang University Email author 
  • , Kangjie ZhuAffiliated withDepartment of Polymer Science and Engineering, Zhejiang University

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


In this study, the concept of hydrophobic ion pairing was adopted for incorporating lysozyme into electrospun poly(ε-caprolactone) (PCL)/poly(ethylene glycol) (PEG) non-woven membranes. The solubility of lysozyme in organic solvent was enhanced through the formation of lysozyme–oleate complexes, which could be directly loaded into PCL/PEG membranes using electrospinning technique. The resultant PCL/PEG nanofibers have a compact structure with an average diameter ranged from about 0.4 μm to 0.9 μm. The addition of PEG into the PCL nanofibers not only improved the hydrophilicity of the membrane, but also played an important role on in vitro lysozyme release rate. It was found that the release rate of lysozyme was enhanced with the increase of PEG content. In addition, the increase of salt concentration in the release medium accelerated lysozyme release. It has also been shown that the released lysozyme retained most of its enzymatic activity.