Journal of Materials Science: Materials in Medicine

, Volume 17, Issue 1, pp 33–41

Observation and quantitative analysis of rat bone marrow stromal cells cultured in vitro on newly formed transparent β-tricalcium phosphate

  • Noriko Kotobuki
  • Daisuke Kawagoe
  • Daishirou Nomura
  • Youichi Katou
  • Kaori Muraki
  • Hirotaka Fujimori
  • Seishi Goto
  • Koji Ioku
  • Hajime Ohgushi
Article

DOI: 10.1007/s10856-006-6327-1

Cite this article as:
Kotobuki, N., Kawagoe, D., Nomura, D. et al. J Mater Sci: Mater Med (2006) 17: 33. doi:10.1007/s10856-006-6327-1

Abstract

To observe living cell morphology on ceramics by light microscopy, we fabricated a new material—transparent β - tricalcium phosphate (t-β TCP) ceramic—for the purpose of serving as a tissue culture substrate. Bone marrow stromal cells (BMSCs) were obtained from rat femora and cultured on both t-β TCP ceramic disks and culture grade polystyrene (PS) dishes in an osteogenic medium. After 1 day of culture, cell attachment and spreading on both the t-β TCP and PS substrata were equally and clearly detected by ordinary light microscopy. After 14 days of culture, extensive cell growth, alkaline phosphatase (ALP) staining, and bone mineral deposition could be detected on both substrata. In addition, quantitative biochemical analyses revealed high DNA content, ALP activity, and osteocalcin content of these cultures. This experiment is significant in that all of the results were similarly observed on both the t-β TCP and PS substrata, indicating the excellent properties of β TCP ceramics for BMSCs culture towards osteogenic differentiation.

Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • Noriko Kotobuki
    • 1
  • Daisuke Kawagoe
    • 2
  • Daishirou Nomura
    • 3
  • Youichi Katou
    • 1
  • Kaori Muraki
    • 1
  • Hirotaka Fujimori
    • 3
  • Seishi Goto
    • 3
  • Koji Ioku
    • 2
  • Hajime Ohgushi
    • 1
  1. 1.Research Institute for Cell Engineering (RICE)National Institute of Advanced Industrial Science and Technology (AIST)AmagasakiJapan
  2. 2.Graduate School of Environmental StudiesTohoku UniversityAoba-kuJapan
  3. 3.Division of Applied Medical Engineering Science, Graduate School of MedicineYamaguchi UniversityYamaguchiJapan