Journal of Materials Science: Materials in Medicine

, Volume 19, Issue 2, pp 789–795

Latex of immunodiagnosis for detecting the Chagas disease: II. Chemical coupling of antigen Ag36 onto carboxylated latexes

  • Verónica D. G. Gonzalez
  • Luis M. Gugliotta
  • Carla E. Giacomelli
  • Gregorio R. Meira
Article

DOI: 10.1007/s10856-006-0041-x

Cite this article as:
Gonzalez, V.D.G., Gugliotta, L.M., Giacomelli, C.E. et al. J Mater Sci: Mater Med (2008) 19: 789. doi:10.1007/s10856-006-0041-x

Abstract

A novel immunodiagnosis reagent for detecting the Chagas Disease was developed, by chemical coupling of antigen Ag36 of Trypanosoma cruzi onto two (carboxylated and core-shell) latexes. The coupling reactions involved the use of a carbodiimide intermediate. Bovine serum albumin (BSA) was used as a model protein for determining the appropriate conditions for its physical and chemical coupling. BSA showed an increased adsorption onto the base carboxylated latexes, with respect to a PS latex without carboxyl groups. The chemical bonding experiments only involved the carboxylated latexes. With BSA, the final density of covalently bound protein was 2.30 mg/m2. In addition, around 55% of the total linked protein was chemically coupled, and the reaction was little affected by the pH. With Ag36, the final density of covalently bound protein was 2.44 mg/m2, around 80% of the total linked protein was chemically coupled, and the chemical coupling was maximum at pH = 5 (i.e., close to the isoelectric point).

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Verónica D. G. Gonzalez
    • 1
  • Luis M. Gugliotta
    • 1
  • Carla E. Giacomelli
    • 2
  • Gregorio R. Meira
    • 1
  1. 1.INTEC (Universidad Nacional del Litoral and CONICET)Santa FeArgentina
  2. 2.INFIQC, Departamento de Fisicoquímica, Facultad de Ciencias QuímicasUniversidad Nacional de CórdobaCordobaArgentina