Journal of Materials Science

, Volume 48, Issue 11, pp 3913–3925

Evaluation of biotinylated magnetic nanoparticles for tumour imaging

Authors

  • Ram Prakash Chauhan
    • Division of Cyclotron and Radiopharmaceutical SciencesInstitute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization
    • Department of ChemistryPanjab University
    • Department of ChemistryS.V.G.C. Ghumarwin
    • Division of Cyclotron and Radiopharmaceutical SciencesInstitute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization
  • Gurjaspreet Singh
    • Department of ChemistryPanjab University
  • Narmada Bag
    • Division of Cyclotron and Radiopharmaceutical SciencesInstitute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization
  • Sweta Singh
    • Division of Cyclotron and Radiopharmaceutical SciencesInstitute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization
  • Krishna Chuttani
    • Division of Cyclotron and Radiopharmaceutical SciencesInstitute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization
  • B. S. Hemanth Kumar
    • Division of NMRInstitute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization
  • Satyam K. Agrawal
    • Department of PharmaceuticalsNational Institute of Pharmaceutical Education & Research
  • Anil K. Mishra
    • Division of Cyclotron and Radiopharmaceutical SciencesInstitute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization
Article

DOI: 10.1007/s10853-013-7193-0

Cite this article as:
Chauhan, R.P., Mathur, R., Singh, G. et al. J Mater Sci (2013) 48: 3913. doi:10.1007/s10853-013-7193-0
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Abstract

Target-specific nanoparticles with good stability, dispersibility and targeting properties are very desirable for in vivo applications like diagnosis and therapy. In this work, the potential of covalently biotinylated magnetic nanoparticles for targeting tumours has been analysed by various in vitro and in vivo studies. For quantitatively estimating the amount of biotin bound to the surface of magnetic nanoparticles, 4-Hydroxyazobenzene-2-carboxylic acid dye was used. Varying the pH conditions changes, the mean size and zeta potential and transverse relaxivity slightly, affects the contrast enhancing capability of the synthesized biotinylated magnetic nanoparticles minimally. Cytotoxicity of the synthesized nanoconjugate was analysed in two different cell lines HeLa and A549 by using sulpho-rhodamine B assay and tumour cell-targeting capability was analysed in HeLa cell lines by using confocal microscopy. The results of cytotoxicity analysis combined with intracellular uptake, biodistribution and gamma scintigraphy prove the tumour-targeting potential of the synthesized biocompatible biotinylated magnetic nanoparticle conjugate.

Copyright information

© Springer Science+Business Media New York 2013